摘要
建立大鼠大肠癌动物模型,选用一系列生物学标志物,观察茶对大肠癌的化学预防效果。结果表明,与阳性对照组相比,在16和32周,绿茶组和茶色素组动物大肠细胞增殖细胞核抗原(PCNA)标记指数和核仁组成区嗜银蛋白(AgNORs)颗粒数目显著减少,ras-p21蛋白表达也降低(P<0.01);而且,绿茶和茶色素还抑制了Bcl-2蛋白的表达,诱导了Bax蛋白的表达(P<0.05或P<0.01)。本研究为PCNA、AgNORs、Bcl-2蛋白、Bax蛋白以及ras-p21蛋白等作为大肠癌人群化学预防研究的生物学标志物的可行性和有效性提供了依据。
The present study was to investigate the chemopreventive effects of tea on colorectal cancer with a series of biomarkers.The results showed that the number of silver stained nucleolar organizer dots per nucleus(AgNORs),labeling index(LI) of proliferating cell nuclear antigen(PCNA)of intestinal mucosa,and the expression of ras p21 protein were significantly reduced in the tea treated groups( P <0 01) as compared with the positive control group.Furthermore,tea and tea pigments inhibited the expression of Bcl 2 protein and induced the expression of Bax protein( P <0 05 or P <0 01).The study provided evidence supporting that PCNA LI,AgNORs,Bcl 2,Bax and ras p21 protein could be used as effective biomarkers for colorectal carcinogenesis in human chemopreventive trials.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第2期109-111,共3页
Journal of Hygiene Research
关键词
生物学标志物
大肠癌
化学预防
biomarker, colorectal cancer, chemoprevention
