摘要
目的 探讨脾脏在仓鼠到大鼠异种肝移植中的体液免疫、细胞免疫作用。方法 三袖套法建立仓鼠到大鼠原位肝移植模型 ,观察移植术后肝脏和脾脏的病理变化 ;采用补体介导的细胞毒实验检测异种肝移植术后血浆中抗仓鼠抗体滴度的变化 ;运用免疫组织化学的方法检测脾脏中大鼠抗体的产生、脾脏增殖细胞核抗原 (PCNA)的表达 ;并观察环孢素A、环磷酰胺、切脾对移植术后生存时间及免疫排斥反应的影响。结果 异种肝移植术后免疫排斥反应发生时 ,脾脏急剧增大 ,边缘区明显增厚 ,产生大量抗体 ,血浆中抗体滴度显著增高 ,移植物遭受破坏。环磷酰胺可选择性抑制脾脏边缘区的增殖 ,明显降低血浆抗体滴度 ;切脾 +环孢素A可抑制其免疫排斥反应 ,生存时间显著延长 (P <0 .0 1) ,但延迟 3d切脾则不能延长其生存时间 (P >0 .0 5 )。结论 在异种肝移植免疫排斥反应中 ,介导体液免疫的抗体主要来源于脾脏边缘区的免疫细胞 ,术后 3d即已到达免疫反应场所 ,在其细胞免疫中发挥重要作用。
Objectives To study the role of spleen in liver xenogenic rejection of Hamster to Rat.Methods The in situ liver transplantation model of hamster to rat was developed by using three cuff technique to observe the pathological change of graft and spleen in xenogenic rejection.The production of antibody of rat and the expression of PCNA in spleen were detected by using immunohistochemistry. In addition, the effects of several immunosuppression measures on the survival time and rejection after transplantation were observed.Results In xenogenic rejection, the spleen proliferated rapidly, its marginal zone obviously thickened and the titer of antibody in serum was increased rapidly. The grafts were injured. The cyclophosphoramide can selectively inhibit the proliferation of marginal zone of the spleen and reduce greatly the titer of antibody in serum. Cyclosporine A plus splenectomy can suppress the xenogenic rejection and prolong the recipient's survival time ( P < 0.01 ), but the time of splenectomy was delayed 3 days, the rejection cann't be suppressed, and the recipient's survival time cann't be prolonged markedly ( P > 0.05 ).Conclusions In concordant liver xenograft, the antibody mediating humoral immunity come mainly from the marginal zone of spleen. The lymphocytes mediating cellular immunity from the spleen located at the area of immunine rejection at third postoperative day.
出处
《中华器官移植杂志》
CAS
CSCD
2000年第2期83-85,共3页
Chinese Journal of Organ Transplantation
