摘要
采用硫酸甲酯化前处理,结合气相色谱-质谱(GC/MS)分离分析技术,研究了烤烟不同叶位叶片(L8-L12,即从底部第8位叶向上到第12位叶)有机酸含量的差异。GC/MS定量分析结果表明:烤烟不同叶位叶片中,苹果酸、γ-戊酮酸、柠檬酸、丙二酸、乙二酸、亚麻酸和软脂酸含量相对较高,是烤烟中主要的有机酸成分;并且不同叶位叶片有机酸、苹果酸与其他有机酸含量差异明显;不同叶位叶片有机酸总量表现为:L8>L9>L12>L10>L11;L8-L12叶片中,非挥发性有机酸、高级饱和脂肪酸、高级不饱和脂肪酸总量分别在有机酸总量中所占比例范围是:88.49%～94.38%,3.01%～5.10%,2.61%～6.40%。不同叶位叶片有机酸含量差异分析结果表明:L10和L12位叶有机酸含量适中,具有优质、高档烟原料的特质。
The pre-treatment of sulfuric acid methyl ester with gas chromatography-mass spectrometry（GC/MS） analysis techniques was utilized to study the difference of the organic acid content in different flue-cured tobacco leaf（L8-L12： from the bottom of the eighth leave up to the twelfth leave）and its influence on fluecured tobacco＇ quality.The results showed that malic acid,γ-e ketone acid,citric acid,malonic acid,linolenic acid and palmitic acid had a higher content relatively in different flue-cured tobacco leaves and they were the main acidic components,what＇s more,the difference between organic acids,malic acid and other organic acids content in different leaf was significantly obvious.The differencce of total organic acids content from the eighth to the twelfth leave was L8〉L9〉L12〉L10〉L11;The content proportion ranges of non-volatile acid,senior saturated fatty acid and senior unsaturated fatty acid in total organic acids were 88.49%-94.38%,3.01%-5.10% and 2.61%-6.40%,respectively.The difference analysis of organic acids content in different leaf position indicated that the organic acids content in the eighth and twelfth leaves was moderate and they could be considered as the superior and slap-up tobacco raw materials.
出处
《华北农学报》
CSCD
北大核心
2011年第4期111-114,共4页
Acta Agriculturae Boreali-Sinica
基金
河南省烟草专卖局资助项目(HYKJ200701
HYKJ200909)
关键词
烤烟
叶位
有机酸
差异分析
Flue-cured tobacco
Leaf position
Organic acid
Variance analysis
