摘要
通过花粉管通道法将ZmPti1,ZmPti1-1,ZmCIPK2三种蛋白激酶基因植物表达载体分别导入玉米自交系吉444,经草丁膦筛选后得40株抗性植株,通过PCR检测其中28株为PCR阳性植株,平均转化率为1.33%,最后收获11株转基因植株。干旱条件下通过对转基因T1植株的单株籽粒产量、千粒重两个指标进行差异显著性和耐旱系数的分析,结果表明,转基因植株与非转基因植株对干旱的敏感性不同,初步认为转基因植株具有抗旱性,表明蛋白激酶能够提高玉米的耐旱性。
Three plant expression vectors harboring protein kinase genes of ZmPti1,ZmPti1-1,or ZmCIPK2 were transferred into maize inbred line Ji 444 separately by pollen-tube-pathway,and 40 glufosinate-tolerant plants were obtained through herbicide screening,then 28 of them were confirmed positive by PCR analysis.The average transformation rate was 1.33%,and 11 transgenic lines were harvesting T1 seeds.Control plants along with T1 transgenic plants were cultivated under drought conditions,and the yield of each plant and 1000-grains weight were analysis,then their drought tolerance coefficient of T1 transgenic lines was calculated,and the elementary results suggested that transgenic plants was more tolerant to drought stress than that of control plants,and protein kinase can improve the drought tolerance of maize.
出处
《华北农学报》
CSCD
北大核心
2011年第4期46-49,共4页
Acta Agriculturae Boreali-Sinica
基金
国家转基因重大专项重点课题(2009ZX08003-009B)
北京市科委项目(Z090605006009014
Z09090501040902)
北京市农林科学院科研能力创新项目
关键词
蛋白激酶
花粉管通道法
转基因玉米
抗旱性
Protein kinase
Pollen-tube-pathway
Transgenic maize
Drought tolerance
