摘要
利用已克隆植物抗病基因NBS(Nucleotide binding site)序列中的保守结构P-loop和GLPL合成简并引物,以小麦近等基因系TcLr24基因组DNA为模板进行PCR扩增。得到13条具有连续ORF的抗病基因类似物(Resistancegene analogues,RGAs)序列,它们之间相应推测的氨基酸序列间的相似性系数在36.9%～98.3%之间。对甘薯RGAs和4个已克隆植物NBS的氨基酸序列进行结构分析表明,它们包括P-loop、Kinase-2、Kinase-3a、GLPL抗病基因所共有的保守结构。同时对分离的RGAs的氨基酸序列进行系统发育树分析,这些RGAs与Xa1、RPS2聚在一起,并且符合nonTIR RGAs类型。这些结果表明小麦与其他物种的NBS类RGAs可能具有同样的起源和进化机制。
Degenerate primers based on conserved motif（P-loop and GLPL）of the nucleotide binding site（NBS） region from the cloned plant disease resistance genes were used to isolate resistance gene analogues（RGAs） from genomic DNA of wheat.13 RGAs with uninterrupted open reading frames（ORFs） were obtained.Sequence identity among the 13 RGAs deduced amino acid sequences showed identity ranged from 36.9 % to 98.3%.The analysis of RGAs amino acid sequence structures suggested that they contained the domains such as P-loop,Kinase-2,Kinase-3a,and GLPL.The phylogenetic analyses for the deduced amino acids showed that RGAs from wheat were nonTIR type.These results showed that NBS type RGAs isolated from wheat might have the same origin and mechanism of evolution as that in other plants.
出处
《华北农学报》
CSCD
北大核心
2011年第4期23-26,共4页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(30771391)
河北省青年基金项目(2010140)
关键词
小麦
NBS
抗病基因类似物
Wheat
NBS（Nucleotide binding site）
RGA（Resistance gene analogues）
