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小麦NBS类序列的分离与特征分析

Isolation and Characteristic Analysis of NBS-type Resistance Gene Analogues in Wheat
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摘要 [目的]获得小麦近等基因系TcLr24中NBS类抗病基因同源序列。[方法]利用已克隆植物抗病基因NBS序列中的保守结构"P-loop"和"GLPL"合成简并引物,以小麦近等基因系TcLr24基因组DNA为模板进行PCR扩增。[结果]得到13条具有连续ORF的抗病基因类似物序列,包括P-loop、Kinase-2、Kinase-3a、GLPL抗病基因所共有的保守结构,相应推测的氨基酸序列间的相似性系数在36.1%～98.3%,同时对分离的RGAs的氨基酸序列和5个已克隆植物NBS的氨基酸序列进行聚类分析表明,与Xa1、RPS2亲缘关系较近,而与Lr21亲缘关系较远。[结论]TcLr24中NBS类RGAs可能会有与Lr21不同机制的抗病功能。 [Objective] The study aimed to acquire the NBS-type resistance gene analogues in wheat TcLr24.[Method] Degenerate primers based on conserved motif（P-loop and GLPL）of the nucleotide binding site（NBS） region from the cloned plant disease resistance genes were used to isolate resistance gene analogues（RGAs） from genomic DNA of wheat TcLr24.[Result] 13 RGAs with uninterrupted open reading frames（ORFs） were obtained The analysis of RGAs amino acid sequence structures suggested that they contained the domains such as P-loop,Kinase-2,Kinase-3a,and GLPL,while the 13 RGAs deduced amino acid sequences showed identity ranged from 36.1% to 98.3%.Cluster analysis showed that 13 RGAs and RPS2 and Xa1 were clustered into one group.[Conclusion]Results showed that NBS type RGAs isolated from wheat TcLr24 might have different mechanism from Lr21.
作者 张立荣 杨文香 刘大群
机构地区 河北农业大学植物保护学院植物病理系河北省农作物病虫害生物防治工程技术研究中心
出处 《安徽农业科学》 CAS 北大核心 2011年第9期5093-5095,共3页 Journal of Anhui Agricultural Sciences
基金 国家自然科学基金项目(30771391) 河北省青年基金项目(2010140)
关键词 小麦 NBS 抗病基因类似物 Wheat Nucleotide Binding Site Resistance Gene Analogues
分类号 S512.1 [农业科学—作物学]
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参考文献19

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安徽农业科学
2011年 第9期

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