摘要
采用两种抗原 (抗原 B和粗抗原 )通过 EL ISA法检测感染细粒棘球蚴和多房棘球蚴 2～ 3月的小鼠、正常鼠以及棘球蚴病人、非棘球蚴病人和健康人血清抗体。结果表明 ,抗原 B和粗抗原检测 2月细粒棘球蚴病鼠血清抗体阳性率分别为 95 %和 10 0 % (P>0 .0 5 )。检测 3月细粒棘球蚴病鼠血清抗体均为 10 0 % ,检测正常鼠血清的假阳性率分别为 0和 5 %(P>0 .0 5 )。检测 3月泡球蚴病鼠的假阳性率分别为 38.46 %和 84.6 2 % (P<0 .0 1)。抗原 B和粗抗原检测 37例细粒棘球蚴病患者血清抗体的阳性率为 10 0 % ,而检测 6 1例非细粒棘球蚴病患者血清抗体的假阳性率为 6 .6 6 %和 19.6 7% (P<0 .0 5 ) ,检测健康人血清的假阳性率分别是 0和 5 % (P>0 .0 5 )。结果提示 ,抗原 B诊断早期棘球蚴病鼠的敏感性与粗抗原相似 ,但特异性优于囊液粗抗原。
In this study, the mice infected with echinococcosis or alveococcosis for two or three months were diagnosed by antigen B and crude antigen through ENISA and Dot ELISA. So were the patients with hydatidosis and non hydtidosis. The results showed that the positive rates of the two month, mice with echinococcosis were 95% and 100% . The false positive rates of sera against antigen B and crude antigen were 38.46% and 84.64%(P<0.05) respectively in B mice infected with alveococcosis for three months, while that of mormal mice 0 and 5%(P>0.05). Moreover, the positive rates of antigen B and crude antigen were both 100% in 37 patients with hydatidosis . And the false ones were 6.66% and 19.67% respectively in 61 non hydatidosis patients (P<0.05). As for 20 healthy, blood dinors, the corresponding rates were 0 and 5%. The above results indicated that the sensitiveity of antigen B was similar to crude antigen in diagnosis of earlier stage of hydatidosis. But the specificity of antigen B was superior to the latter.
出处
《地方病通报》
2000年第1期20-21,45,共3页
Endemic Diseases Bulletin
关键词
棘球蚴病
早期诊断
抗原B
实验研究
Antigen B
Earlier experimental period
Immunodiagnosis
Echinococcosis mice
ELISA
Dot ELISA
