摘要
给同基因小鼠皮下接种TD。小鼠宫颈癌(U_(14))细胞;然后,给实验组动物腹腔注入不同剂量的抗U_(14)单克隆抗体(AU_(14-1))。结果发现,肿瘤细胞接种后的267天中,对照组动物全部未发生肿瘤,都存活;而在大剂量AU_(14-1)处理的实验组动物中,86—88%在接种部位发生了实体瘤,且死于全身性肿瘤疾患。表明AU_(14-1)对于U_(14)细胞的免疫促进作用。应用AU_(14-1)研究U_(14)细胞在体外对特异抗体的反应,结果证实,AU_(14-1)诱发U_(14)细胞抗原调变。间接膜免疫荧光表明,AU_(14-1)抗体和U_(14)抗原从这些细胞表面消失。本研究结果提示抗原词变可能是宫颈癌细胞逃逸单克隆抗体治疗的重要机制。
The TD。 of mouse uterine cervical carcinoma (U_(14)) were inoculated subcutaneously in syngenic mice. After that experimental mice were administered diverse doses of monoclonal anti-U_(14) antibody (AU_(14-1)). It was found that within a 267 day interval after the tumor cell inoculations, all control animals were tumor free with tumor free survival, but 86% (6/7)and 88% (7/8)of experimental mice that had been treated with high dose AU_(14-1) showed progressive tumor growth at the inoculation site and died of systemic tumor disease. These results indicate immune enhancement of AU_(14-1) on U_(14) cells. AU_(14-1) was used to study the response of U_(14) cells to specific anti (?)dy in vitro. The results demonstrate that AU_(14-1) induces antigenic modulation of U_(14) cells, which is shown to be a loss of AU_(14-1) antibody and U_(14) antigen from the surface membrane of these cells as determined by indirect membrane immunofluorescence assay. This suggested that antigenic modulation may be proposed as a mechanism by which cervical cancer cells escape monoclonal antibody therapy.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1990年第3期160-163,共4页
Chinese Journal of Pathophysiology
关键词
宫颈癌
单克隆抗体
抗原
Antibodies, monoclonal
Antigens
Cervix neoplasms
Mice
