摘要
本实验采用正丁醇提取方法以研究甲基胆蒽诱发的小鼠宫颈癌(U_(14))细胞表面抗原。用2.5%正丁醇溶液对U_(14)细胞进行提取所获得的正丁醇粗提物(CBE),经Lowry法测得其蛋白含量为147微克/毫升;在SDS-PAGE中显出8条区带,分子量为18-70kD;用ELISA检测表明CBE与抗宫颈癌单克隆抗体(AU_(14-1))和多克隆抗体均呈阳性反应。结果证明CBE系低分子量的宫颈癌细胞表面抗原,其中含有能够与AU_(14-1)发生免疫反应的肿瘤抗原决定簇。
Cervical cancer (U_(14)) cell surface antigen induced by methylcholanthrene was extracted by 1-butanol. The U_14 cells were incubated in 2. 5% 1-butanol, the resul-ted supernatants were referred to as CBE (crude butanol extract). Protein concentration ofCBE determined by the Lowry assay was 147μg/ml. CBE was characterized by SDS-PAGE, which showed eight bands on gel. Their molecular weight range was found to be18-70 kD. CBE was tested by indirect ELISA, which showed that CBE had positive reac-tion with both monoclonal (AU_(14-1)) and polyclonal antibodies. These results indicatedthat CBE contained low molecular cervical cancer cell surface antigen and the antigenicdeterminant which binds to AU_(14-1) specifically.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1993年第1期40-43,共4页
Chinese Journal of Pathophysiology
关键词
抗原
子宫肿瘤
子宫颈肿瘤
正丁醇
Antigens,neoplasm
Cervix neoplasms
Alconols,butyl
Antibodies,monoclonal
Mice
