摘要
目的:探讨槲皮素对血管内皮细胞损伤的保护作用及机制。方法:采用体外培养的人脐静脉内皮细胞株ECV304,观察内皮细胞加入槲皮素8,4,2 mg.L-1培养24 h后,再加入H2O2培养18 h后,造成血管内皮细胞损伤模型。用流式细胞仪测定内皮细胞死亡率,用ELISA法检测内皮细胞培养液血栓调节蛋白(TM)的含量;荧光分光光度法测定内皮细胞培养液中乳酸脱氢酶(LDH)的活性。结果:与H2O2损伤后的模型组相比,槲皮素可以减低细胞死亡率,[模型组细胞死亡率为(28.36±0.10)%,槲皮素高、中、低剂量分别为(8.34±0.01)%,(16.58±0.04)%,(10.12±0.02)%],可使培养液中TM蛋白含量减少,[模型组为(58.5±18.8)μg.L-1,槲皮素高、中、低剂量为(32.7±9.7),(27.8±1.9),(32.1±8.6)μg.L-1]和LDH活性减少[模型组为(1 931.9±159.5)U.L-1,槲皮素高、中、低剂量为(1.092±126.2),(1 159.6±274.2),(1 223.5±120.5)U.L-1]。结论:槲皮素能明显保护过氧化氢对内皮细胞的损伤,其保护作用与抗脂质过氧化、保护细胞的完整性有关。
Objective：To evaluate the effects of quercetin on the injured vascular endothelial cells and its mechanism.Method：Endothelial cell culture was in vitro.The endothelial cell injury model was established by cultured with H2O2 for 18 h,Quercetin at dose of 8,4,2 mg·L-1 was cultured with the cells before the model establishment.Flow cytometry was used for determining endothelial cell death rate.Endothelial cell was detected by ELISA for medium thrombomodulin（TM） content;spectrophotometric determination was used for the culture medium lactate dehydrogenase（LDH） activity.Result：Compared to the model group,quercetin can lower endothelial cell death rate,reduce the content of TM and activity of LDH in the culture medium.Conclusion： Quercetin can obviously protect endothelial cells,and its protective effect is related to against lipid peroxidation,protection of the cell membrane.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第5期174-176,共3页
Chinese Journal of Experimental Traditional Medical Formulae
