摘要
目的研究社区相关性耐甲氧西林金黄色葡萄球菌(CA-MRSA)的分子生物学特性,为防控CA-MRSA感染提供依据。方法收集2007年1月-2008年9月间分离的耐甲氧西林金黄色葡萄球菌共5株,采用PCR方法检测进行SCCmec分型,多位点序列分型(MLST),葡萄球菌A蛋白(SPA)分型。结果 5株CA-MRSA菌株SCCmecⅣ基因分型为3株,SCCmecⅤ型2株;MLST分型为SCCmecⅣ型菌株均为ST59型,SCCmecⅤ型中1株为ST7型,另1株未能成功进行MLST分型;SPA基因分型将3株SCCmecⅣ型菌株归属为t437,另2株分别为t163和t796。结论流行传播的CA-MRSA感染主要以SCCmecⅣ型菌株为主,应引起密切关注,并做好防控工作。
Objective The research of the molecular biology characteristics for community associated methicillin-resistant Staphylococcus aureus（CA-MRSA）,provide a basis for clinical prevention and control of CA-MRSA infection.Methods Collected five methicillin-resistant Staphylococcus aureus between January 2007 to September 2008,Using PCR for Staphylococcus aureus gene cassette（SCCmec） typing and multi-locus sequence analysis（MLST） and Staphylococcus A protein（SPA） typing.Results The three SCCmecⅣ of 5 CA-MRSA and another two was SCCmecⅤ type.MLST typing of SCCmecⅣ type strains were ST59-type and SCCmecⅤ type strains were ST7-type and Another one failed to MLST type.SPA genotyping of the three SCCmecⅣ strain attributed to the t437,the other two were t163 and t796.Conclusion Prevalent CA-MRSA infection mainly carry the gene-based SCCmecⅣ strain.This study shows that exists CA-MRSA infection in the clinical,so should pay a close attention,make perfect job of monitoring the CA-MASA infection.
出处
《中国实验诊断学》
2012年第2期292-295,共4页
Chinese Journal of Laboratory Diagnosis
基金
辽宁省科学技术计划项目(2008225010-10)
