摘要
了解我院患者耐甲氧西林金黄色葡萄球菌（MRSA）的分子流行病学特点,为临床抗感染治疗提供依据。收集2007年1月~2008年9月我院分离的耐甲氧西林金黄色葡萄球菌共54株,采用PCR进行SCCmec基因分型、葡萄球菌A蛋白（SPA）分型,并检测杀白细胞毒素（PVL）基因,同时应用脉冲场凝胶电泳（PFGE）进行同源性分析。54株MRSA菌株SCCmec基因分型为SCCmecⅡ型17株,SCCmecⅢ型33株,SCCmecⅣ型2株,SCCmecⅤ型2株;SPA基因分型将28株归属为t030,9株为t002,8株为t037,5株为t570,2株为t437,t163和t796各1株;PVL毒素检测只有2株SCCmecⅣ型菌株阳性;PFGE证实院内MRSA感染主要为2种克隆株传播,同时还有其他型别出现。本院MRSA流行传播的SCCmec基因型主要以Ⅲ型占优势,同时发现有携带PVL毒素的CA-MRSA分离株流行,应引起密切关注。
Molecular epidemiological characteristics of patients suffered from methicillin-resistant Staphylococcus aureus（MRSA） in the hospital were penetrated in order to provide foundation for anti-infective therapy.54 strains of MRSA isolated in the hospital during January 2007~September 2008 were collected and adopted polymerase chain reaction（PCR） carried out SCCmec and Staphylococcus A protein（SPA） genotyping,as well as Panton-Valentine leukocidin（PVL） gene detection,and pulsed-field gel electrophoresis（PFGE） homology analysis.The results showed that SCCmec gene typing among 54 MRSA strains SCCmecⅡwas 17 strains,SCCmecⅢ 33 strains,SCCmecⅣ 2 strains,SCCmecⅤ2 strains;and SPA genotyping get 28 MRSA attributed to t030,9 strains to t002,8 strains to t037,5 strains to t570,2 strains to t437,and each to t163,t796.The PVL toxin detection only two SCCmecⅣ strains were positive;Nosocomial infection of MRSA was confirmed primarily spread by two cloned strains which were proved by PFGE,while occurred other types emerged.Therefore,the prevalence and spread of MRSA in the hospital mainly dominated by SCCmecⅢ genotype;And it was found at the same time that PVL toxin-carrying isolates of CA-MRSA was epidemic that should be paid a close attention to.
出处
《微生物学杂志》
CAS
CSCD
2011年第1期34-38,共5页
Journal of Microbiology
基金
辽宁省科技计划项目(2008225010-10)
关键词
耐甲氧西林金黄色葡萄球菌
基因分型
杀白细胞毒素
methicillin-resistant
Staphylococcus aureus
genotyping
Panton-Valentine leukocidin
