摘要
目的研究高同型半胱氨酸血症对骨骼肌细胞GLUT4表达和膜分布的改变,探讨同型半胱氨酸对葡萄糖摄取的影响.方法 20只6周健康小鼠随机分为对照组(10只)和高同型半胱氨酸血症组(10只),含1.5%的蛋氨酸饮水3个月制造高同型半胱氨酸血症模型;测定空腹状态下血糖和胰岛素;HE染色观察骨骼肌组织形态改变;免疫组织化学和Western Blot检测骨骼肌细胞GLUT4表达和细胞膜上分布.结果高同型半胱氨酸血症组与对照组比较空腹血糖和空腹胰岛素增加(P<0.05),骨骼肌组织结构完整,细胞膜上GLUT4分布降低,GLUT4蛋白质表达无明显差异(P>0.05).结论在骨骼肌组织中,同型半胱氨酸可能通过抑制GLUT4在细胞膜上分布,降低骨骼肌细胞对葡萄糖的摄取.
Objective To study the variations in the expression and distribution of Glucose transporter 4 （GLUT4） in skeletal muscle cells in hyperhomocysteinemia animal model, to explore effect of hyperhomocysteinemia on glucose uptake. Methods Twenty healthy mice, six weeks of age, were randomly divided into control group （n = 10） and hyperhomocysteinemia group （n = 10）. Normal food was fed in control group; Normal food and 1.5 % （mass fraction） methionine were fed in hyperhomocysteinemia group. After the feed in the hyperhomocysteinemia group and the control group were fed for three months, the levels of blood glucose and blood insulin were determined in the fasting. The expression and distribution of GLUT4 were observed with [mmunohistochemistry and Western Blot in skeletal muscle cells. Results The fasting plasma glucose and plasma insulin levels in the hyperhomocysteinemia group were higher than those in the control group （P 〈 0.05 ）. Strncture of skeletal muscle tissue were intact. The quantity of GLUT4 distribution in the cytomembrane was decreased in the herpyhomocysteinemia group compared with the control group. However, there were no significant difference in expression of the GLUT4 between the control group and herpyhomocysteinemia group （P 〉 0.05）. Conclusion Homocysteine may suppress distribution of GLUT4 in the cytomembrane in skeletal muscle cells, resulting inreducing glucose uptake in skeletal muscle cells.
出处
《昆明医学院学报》
2012年第1期16-19,共4页
Journal of Kunming Medical College
基金
云南省科技厅-昆明医学院联合专项基金资助项目(2009CD213
2010CD223)
云南省应用基础研究面上资助项目(2010CD075
2007CO70M)
云南省教育厅基金资助项目(2010C148)
