摘要
【目的】研究木枣枝条和不同处理枣吊的挥发性组分和含量,分析枣镰翅小卷蛾对不同处理枣吊的行为反应,为生产上应用植物源引诱剂防治枣镰翅小卷蛾提供理论依据。【方法】利用固相微萃取技术(SPME)和GC-MS,分析木枣枝条和不同处理枣吊的挥发物成分,利用触角电位技术(EAG)和Y-嗅觉仪,测定枣镰翅小卷蛾的电生理及行为反应。【结果】木枣枝条的挥发物成分有9种,主要挥发性组分为萜类和酯类化合物;木枣不同处理枣吊共有的挥发物成分有17种,多为萜类、酯类和醇类化合物。健康枣吊、人工机械损伤枣吊和虫害枣吊相比,三者在挥发性组分组成及比例上均存在差异,并且在遭受虫害后,木枣枣吊可释放出虫害诱导型挥发物乙酸-4-己烯-1-醇酯。枣镰翅小卷蛾成虫对木枣的触角电位(EAG)反应表明,枣镰翅小卷蛾成虫对枝条有强烈的刺激反应,且雌虫的反应值显著或极显著高于雄虫。行为测试结果显示,当木枣枣吊受到机械损伤后,其对枣镰翅小卷蛾的趋向选择产生了驱避行为,而健康和虫害枣吊则具有明显的吸引作用。【结论】木枣不同部位释放的挥发物成分并不相同,在健康和虫害枣吊所释放的挥发物成分中,含有对枣镰翅小卷蛾起引诱作用的成分。
【Objective】The volatile composition and content from Ziziphus jujuba Mill.cv.Muzao,and the behavioral response of adult Ancylis sativa to shoot with different treatments were studied in order to provide theoretical basis in biological control for the A.sativa.【Method】Solid phase microextraction（SPME） and gas chromatography-mass spectrometry（GC/MS） were used to analyze the volatile composition.Eelectroantennogram and behavioral responses of adult A.sativa were determined by electroantennogram recording technique and Y-tube olfactometer.【Result】The volatile compounds of branch of Z.jujuba Mill.cv.Muzao were 9 compounds that it comprised mainly a range of terseness and esters.The volatile compounds of shoots of Z.jujuba Mill.cv.Muzao were 17 compounds,and consisted of a range of terseness and esters,secondly alcohol.The volatiles composition and ratio of healthy,artificial and infested damage shoots were detected different.4-hexen-1-ol,acetate is an herbivore-induced plant volatile which is emitted rapidly after leaf damaged and may attract natural enemies.EAG response of A.sativa on Z.jujuba Mill.cv.Muzao showed that it had strong response in millivolts,and the absolute response in millivolts of the female antenna was in general substantially higher than the male.The results of olfactory experiment demonstrated that the artificial shoot showed significant repellency against moth of A.sativa while the shoots whatever healthy and infested damage showed attraction.【Conclusion】The volatile composition from Z.jujuba Mill.cv.Muzao with different parts were not the same,there were attraction compounds to adult A.sativa in the volatiles of healthy and infested damage shoots.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2012年第1期71-78,共8页
Journal of Northwest A&F University(Natural Science Edition)
基金
农业部公益性行业专项(201103024)
长江学者和创新团队发展计划项目(IRT1035)
关键词
枣镰翅小卷蛾
触角电位反应
枣树挥发物
行为反应
Ancylis sativa Liu
electroantennogram responses
volatiles of Ziziphus jujuba Mill
behavioral responses
