摘要
采用动态顶空采样法结合气相色谱-质谱分析技术(GC-MS)研究受榆紫叶甲成虫危害的家榆挥发物的变化情况,并利用触角电位和"Y"型嗅觉仪比较分析榆紫叶甲雌雄成虫对主要成分的触角电生理和行为反应特点。结果表明:受榆紫叶甲成虫危害的家榆释放19种成分,其中顺-3-己烯醇、α-蒎烯、柠檬烯、顺-3-乙酸叶醇酯、芳樟醇、水杨酸甲酯、石竹烯、α-法尼烯和(E,E)-4,8,12-三甲基-1,3,7,11-十三碳四烯(TMTT)9种化合物与健康家榆相比有显著差异。除了TMTT,对其余8种化学标准品进行生测的结果表明:雌性成虫对顺-3-己烯醇、芳樟醇和α-法尼烯有较强的趋性反应,而雄性成虫对α-法尼烯和石竹烯的趋性反应较强。这可为应用引诱剂进行田间监测和控制榆紫叶甲的危害提供理论依据。
Herbivore-induced plant volatiles（HIPVs） from Ulmus pumila were collected by a dynamic headspace sampling method,and analyzed by using GC-MS. Furthermore,electroantennogram（EAG） and behavioral responses of Ambrostoma quadriimpressum（Coleoptera： Chrysomelidae） to the main compounds of HIPVs were investigated. Volatile analyses showed that infestations of A. quadriimpressum markedly increased the emission of 9 compounds,α-farnesene,caryophyllene,cis-3-hexenol,α-pinene,limonene,linalool,methyl salicylate,cis-3-hexenyl acetate and TMTT. The results of behavioral bioassays by using 8 synthetic compounds（except for TMTT） revealed that adult females had a stronger response to linalool,α-farnesene and cis-3-hexen-1-ol than to other compounds tested;and adult males had a stronger response to α-farnesene and caryophyllene. EAG results were basically in accordance with Y-tube olfactometer bioassay. These results may provide practical applications for monitoring and controlling A. quadriimpressum populations in the field.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2010年第10期76-82,共7页
Scientia Silvae Sinicae
基金
吉林省科技发展计划项目(20080234)
关键词
虫害诱导挥发物
榆紫叶甲
质谱分析
触角电位反应
行为反应
herbivore-induced plant volatiles
Ambrostoma quadriimpressum
GC-MS
EAG responses
behavioral response
