摘要
目的检测和分析不同进展阶段的HIV-1感染者外周血T细胞、B细胞和NK细胞亚群变化。方法用全自动血液分析仪和流式细胞仪分别检测253例HIV．1感染者血常规和外周血CD3＋T细胞、CD4＋T细胞、CD8＋T细胞、CD19＋B细胞、CD16＋CD56＋NK细胞亚群，其中CD4＋T细胞数超过500个／止37例（A组），在200～500个／止之间的152例（B组），低于200个／止64例（C组）。对照组为40例排除HIV、HCV、HBV感染的健康人（N组）。多组比较采用单因素方差分析。结果A、B、C三组中，CD4＋T细胞数越低的组，淋巴细胞占白细胞的百分比和总淋巴细胞数也越低，且差异均有统计学意义（PASCN〈0．01）。HIV感染A、B、C组CD3＋T细胞、CD3＋CD8＋T细胞、CD19＋B细胞占淋巴细胞的百分比均高于对照组（PAN〈0．05，PBN〈0．01，PCN〈0．01）；CD4＋T细胞数越低的组，CD3＋T细胞、CD3＋CD8＋T细胞和CD19＋B细胞绝对计数也相应越低，各组相比差异有统计学意义（PABcN〈0．01）。A、B、C三组CD16＋CD56＋NK细胞占淋巴细胞的百分比与NK细胞绝对计数变化呈相反趋势，CIM＋T细胞数越低的组，CD16＋CD56＋NK细胞百分比越高，而其绝对计数越低，且CD16＋CD56＋NK细胞占淋巴细胞的百分比与NK细胞绝对计数均低于正常对照组水平（PABCN〈0．01）。结论HIV-1感染不仅影响人体细胞免疫，可能还影响人体的体液免疫和天然免疫。
Objective To evaluate the changes of peripheral blood T cells, B cells and NK lymphocyte subsets in HIV-1-infected individuals. Methods Blood routine examination by automated hematology analyzer and CD3＋ T cells, CD4＋ T cells, CD8＋ T cells, CD19＋ B cells and CD16＋ CD56＋ NK lymphocyte subsets in peripheral blood by flow cytometry were detected in 253 HIV-1- infected individuals, whom were divided into 3 groups according to CD4＋ T cell counts： more than 500 cells/μL as group A （37 cases）, 200-500 cells/μL as group B （152 cases）, less than 200 eells/ttL as group C （64 cases）. 40 healthy individuals excluded HIV/HCV/HBV infection as control group （group N）. One-Way ANOVA was used for comparison of multi-groups. Results In 3 groups, the lower CD4 ＋ T cell counts, the lower lymphocyte percentage in white blood cells and total lymphocyte counts, and the differences were statistical significant （ P＋BCN 〈 0.01 ）. The percentages of CD3＋ T cells, CD3＋ CD8＋ T cells and CD19＋ B ceils in lymphoeytes were higher than them in group N （ PAN 〈 0.05, PBN 〈 0.01, PCN 〈 0.01, respectively）. The lower CD4＋ T cell counts, the lower cotmts of CD3 ＋ T cells, CD3 ＋ CD8 ＋ T cells and CDI9 ＋ B cells （ PABCN 〈 0.01 ）. The CDI6 ＋ CD56 ＋ NK cell percentage in lymphocytes in group A, B and C was inversely proportional to NK cell counts. The lower CD4 ＋ T cell counts, the higher CD16＋ CD56＋ NK cell percentage, the lower NK cell counts. Moreover, the percentage of CD16＋ CD56＋ NK cells in lymphocytes and the NK cell counts were lower than them in control group（ PAI＋CN 〈 0.01 ）. Conduslons HIV-1 infection not only affects human cell immunity, but also may affect humoral and innate immunity.
出处
《国际流行病学传染病学杂志》
CAS
2012年第1期25-28,共4页
International Journal of Epidemiology and Infectious Disease
基金
广州市科技局应用基础研究项目（2007J1-C0191）
