摘要
目的应用巢式PCR检测恶性疟原虫体外培养中的支原体污染。方法根据支原体16s和23s保守序列设计PCR引物,应用巢式PCR检测恶性疟原虫体外培养中的支原体。结果体外培养的恶性疟原虫标本16份,PCR检测支原体均阳性,经测序比对后确认为口腔支原体。已知阴性对照无扩增带。结论应用巢式PCR能敏感和特异地检出恶性疟原虫体外培养中的支原体。
Objective To detect Mycoplasma contaminants in vitro in cultured Plasmodium falciparum and then to verify the contaminating species.Methods Culture isolates of Plasmodium falciparum were screened for the presence of Mycoplasma using nested PCR with two sets of primers,and the16S-23S rRNA sequence was amplified.Results All 16 isolates were clearly determined to be positive for Mycoplasma according to nested PCR.The sequence of 16S-23S rRNA was compared to the reference sequence for Mycoplasma species.Contaminants in the culture were identified as Mycoplasma orale.Conclusion The results of a nested PCR assay proved to be a sensitive and specific indicator of Mycoplasma contamination in vitro in culturedPlasmodium falciparum.
出处
《中国病原生物学杂志》
CSCD
北大核心
2012年第1期32-33,29,共3页
Journal of Pathogen Biology
关键词
支原体
疟原虫
恶性
培养
巢式PCR
Mycoplasma
Plasmodium falciparum
culture
nested PCR
