摘要
比较研究乳糖化人生长激素 (h GH- L )和人生长激素 (h GH )在小鼠体内药代动力学特征 ,为 h GH- L替代 h GH作为提高治疗侏儒症疗效的可能性提供依据。给小鼠静脉注射 h GH- L后定时取血和肝样 ,用 RIA技术测定血药浓度和肝药浓度 ,然后根据血药浓度 -时间曲线和肝药浓度 -时间曲线分析得出药代动力学参数 ,并与h GH对比研究。结果显示 :h GH- L 的血药浓度 -时间曲线下面积 (AU C为 32 6 86 .9)和在血清的平均驻留时间(MRT为 2 1 .37分钟 )均小于 h GH(AU C为 36 91 3.0 8,MRT为 2 4.98分钟 ) ,表明其在血液的分布量小于 h GH、清除快于 h GH;靶器官肝脏的 h GH- L分布半减期 t1 /2α为 1 .84分钟 ,消除半减期 t1 /2β为 1 1 .0 9分钟 ,肝药浓度 -时间曲线 AUC为 1 76 2 1 .9,而 h GH的 t1 /2α为 2 .1 1分钟 ,t1 /2β为 75 .6 5分钟 ,AU C为 1 2 1 4 8.2 ,表明 h GH- L的肝摄取量大于 h GH,而肝分布和清除均快于 h GH。因此 ,h GH- L 的药代动力学特征明显优于 h GH。
This study was undertaken to explore and compare the pharmacokinetic features of lactosaminated human growth hormone(hGH L) and human growth hormone (hGH). Radioimmunoassay (RIA) technique was used. After mice were intravenously injected with hGH L,their blood and liver samples were collected and handled. The blood drug concentration and liver durg concentration were determined using RIA technique. Then the pharmacokinetic parameters were obtained by analysing the curves of blood drug concentration time and liver drug concentration time. hGH was determinated with the same methods. The results showed that the area under curve (AUC) of blood drug concentration time curve of hGH L was 32 686.90, the mean remain time (MRT) of hGH L in blood was 21.37 minutes, but the AUC of blood drug concentration time curve of hGH was 36 913.08 and the MRT of hGH in blood was 24.98 minutes. Therefore, in blood, the uptake of hGH L was less than that of hGH, the removal of hGH L was faster than the removal of hGH. In liver, for hGH L, the half time of distribution(t 1/2α ) was 1.84 minutes, the half time of removal(t 1/2β ) was 11.09 minutes, the AUC of liver drug concentration time curve was 17 621.9; for hGH, the t 1/2α was 2.11 minutes,the t 1/2β was 75.65 minutes,the AUC of liver durg concentration time curve was 12 148.2. Therefore, in liver, the uptake of hGH L was more than that of hGH, the distribution and removal of hGH L were faster than those of hGH. Therefore, the pharmacokinetic features of hGH L was better than the feature of hGH.
出处
《华西医科大学学报》
CSCD
2000年第1期7-10,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金!资助项目 (批准号 3 9670 861)
关键词
人生长激素
药代动力学
hGH-L
生长激素缺乏症
Lactosaminated human growth hormone\ \ Human growth hormone\ \ Pharmacokinetics Radioimmunoassay
