摘要
目的研究内皮抑素对阿霉素(ADM)诱导的大鼠胶质瘤多药耐药细胞株GL15(GL15/ADM)细胞磷酸糖蛋白(P-gp)表达的抑制作用,探讨其对胶质瘤肿瘤耐药的影响。方法将内皮抑素质粒转染胶质瘤耐药细胞,应用免疫印迹法、荧光分光光度计和流式细胞术分别检测转染后6,12,24,48h不同时间段P-gp表达水平及天冬氨酸特异性半胱氨酸蛋白酶3(caspase-3)的活性和细胞凋亡率。结果转染后6h即可发现P-gp表达水平活性明显降低(P<0.05),且随转染后时间的延长而递减;转染后6h即可发现caspase-3活性明显上升(P<0.05),且随时间的延长而递增;转染后6、12、24、48h肿瘤细胞凋亡率上升,并与非转染组间有极显著性差异(P<0.01)。结论内皮抑素能通过上调Caspase-3的活性,明显抑制胶质瘤耐药细胞中P-gp表达水平和促进肿瘤细胞凋亡,有助于提高胶质瘤的综合治疗效果。
Objective To investigate the effect of endostatin on the expression of phosphoglucoprotein ^（p-gp） in adriamycin （ADM）- induced multidrug resistance glioma cell line GL15 （GL15/ADM） cells in order to explore the role of endostatin in the glioma ＇.~ muhidrug resistance, iMethods The plasmids with endostatin （pcDNA 3.1 ES） were transfected into GL15/ADM cell, in which the expression of P-gp, the activity of cysteine-containing aspartate-specific proteases 3 （caspase-3） and the apoptosis were determined respectively by western blot, spectrofluorometer and flow cytometer 6, 12, 24, and 48 hours after the transfeetion. Results The signifuicnat decrease in the level of P-gp expression in GL15/ADM cells was observed 6 hours after the transfectton and it was positively related to the time after the transfection （P〈0.05）. The signifuicnat increase in the activity of caspase-3 in~ GL15/ADM cells was observed 6 hours after the transfection and it was positively related to the time after the transfection （P〈0.05~）. The apoptosis rates in GL15/ADM cells transfected into by pcDNA 3.1 ES were significance higher 6, !2, 24 and 48 hours after the transfection than those in the untransfected GLIS/ADM cells （P〈0.01）. Conclusions Endostatin can inhibit the level of P-gp expression and promote the apoptosis in GL15/ADM cells by upregulation of the activity of caspase-3, and therefore it is helpful to the increase in the effect of comprehensive treatment on the gliomas.
出处
《中国临床神经外科杂志》
2012年第2期97-99,共3页
Chinese Journal of Clinical Neurosurgery
基金
湖北省卫生厅2008年度青年科技人才基金项目(NO:QJX2008-57)
