摘要
目的探究瘦素受体基因Gln223Arg多态性与上海某区儿童超重肥胖的关系及相关生化指标的相关性。方法采用PCR-RFLP对上海松江区六所小学7-11岁超重肥胖与正常体重儿童(各262名)的血样进行LEPR基因Gln223Arg多态性检测,logistic回归分析其与儿童超重肥胖的相关性,并分析了生化指标在超重肥胖组与正常组儿童以及不同基因型间的差异。结果超重肥胖组与对照组之间LEPR基因Gln223Arg AA频率与A等位基因频率均无统计学差异(P>0.05)。对其收缩压(SBP),舒张压(DBP),胆固醇(TC),甘油三酯(TG),血糖(GLU),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C)进行t检验,得出SBP,DBP,TG,HDL-C和LDL-C在超重肥胖组与对照组中差异显著。在控制了混杂因素BMI等后,LEPR基因Gln223Arg各基因型组的生化指标差异分析表明基因型可能与LDL-C相关(P<0.05)。进一步两两比较结果表明LDL-C水平在基因型AA组比AG(P=0.019)与GG组(P=0.017)低,而AG与GG组之间的差异无统计学意义(P=0.517)。结论 LEPR基因Gln223Arg多态性可能与儿童超重肥胖的发生无关,而LDL-C(P<0.05)可能与LEPR基因Gln223Arg基因型有相关性。
Objective To evaluate the association between leptin receptor（LEPR） Gln223Arg polymorphism and overweight-obesity in children,as well as related biochemical indices.Method The subjects were from six primary schools with the age from seven to eleven,in Songjiang District,Shanghai,China.LEPR Gln223Arg polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism in 262 normal weight subjects and 262 overweight-obese subjects.And logistic regression was used for analysis of the LEPR Gln223Arg polymorphism with obesity.Difference of biochemical indices was analyzed between the two groups,as well as between different genotypes.Results There was no significant difference of AA genotype and A allele of LEPR gene between the overweight-obesity group（1.9%,13.4%） and the normal weight group（0.4%,12.0%）.There were significant differences of SBP,DBP,TG,HDL-C and LDL-C between two groups.The difference of LDL-C level between the genotypes was significant after controlling the confounding factors including BMI.Further analysis revealed that the difference between the genotype AA,AG（P=0.019） and AA,GG（P=0.017）was significant,while the difference between the genotype AG,GG（P=0.517）was insignificant.Conclusion The polymorphism of LEPR gene Gln223Arg may not be associated with overweight-obesity,but may be related to LDL-C.
出处
《营养学报》
CAS
CSCD
北大核心
2012年第1期20-23,共4页
Acta Nutrimenta Sinica
基金
科技部"十一五"国家科技支撑计划(No.2008BAI58B05)
莙政基金中国大学生见习研修计划(No.10040)
关键词
瘦素受体基因
多态性
超重肥胖
生化指标
LEPR gene
polymorphism
overweight-obesity
biochemical indices
