摘要
目的研究瘦素受体基因Ｇｌｎ２２３Ａｒｇ变异与Ⅱ型糖尿病代谢紊乱及其合并高血压的关系。方法运用聚合酶链反应限制性片段长度多态性（ＰＣＲＲＦＬＰ）方法测定无亲缘关系，且有完整临床资料的３５９例上海地区汉人的瘦素受体基因Ｇｌｎ２２３Ａｒｇ变异的基因型（包括１９３例糖耐量正常者及１６６例Ⅱ型糖尿病患者）。局部体脂分布参数由核磁共振仪测定。结果Ⅱ型糖尿病不同性别、不同瘦素受体基因Ｇｌｎ２２３Ａｒｇ变异的高血压合并频率比较发现，瘦素受体基因Ｇｌｎ２２３Ａｒｇ变异与Ⅱ型糖尿病男性合并高血压相关（Ｐ＝０００８）。“Ａ”等位基因与Ⅱ型糖尿病男性较高的收缩压（Ｐ＝０．００２６）及舒张压（Ｐ＝０．００８４）相关。ｌｏｇｉｓｔｉｃ回归分析证实，该基因变异是Ⅱ型糖尿病男性合并高血压的独立风险因子（Ｐ＝０．００３１），且主要与收缩压相关（Ｐ＝０．００５４）。携带“Ａ”等位基因的Ⅱ型糖尿病男性其高血压发生的比数比（ＯＲ）为２．８２５（９５％可信限为１．４１８、５．６２７）。结论瘦素受体基因Ｇｌｎ２２３Ａｒｇ变异与Ⅱ型糖尿病男性合并高血压相关，且主要表现与收缩压相关。
Objective To investigate the association of Gln223Arg variant in leptin receptor gene with metabolic abnormalities and hypertension in type Ⅱ diabetes mellitus. Methods The genotypes of Gln223Arg variant in leptin receptor gene were determined by polymerase chain reaction restriction fragment length polymorphisms (PCR RFLP) assay in 359 unrelated subjects of Shanghai “Han” population (including 193 subjects with normal glucose tolerance and 166 type Ⅱ diabetic patients). The clinical data were also analyzed. The parameters for regional adipose tissue distribution were measured by magnetic resonance imaging (MRI). Results Significant difference of genotype frequency of Gln223Arg variant in leptin receptor gene was observed between hypertensive and normotensive type Ⅱ diabetic male patients ( P =0.008). “A” allele was associated with increased systolic blood pressure ( P =0.0026) and diastolic blood pressure ( P =0.0084) in type Ⅱ diabetic male paitents. Logistic regression analysis showed that this gene variant was an independent risk factor of type Ⅱ diabetic male patients accompanied with hypertension ( P =0.0031),and it was associated with elevation of systolic blood pressure ( P =0.0054) . The odds ratio of hypertension in type Ⅱ diabetic male patients with “A” allele was 2.825 (95% CI 1.418,5.627). Conclusion Gln223Arg variant in leptin receptor gene is associated with hypertension in type Ⅱ diabetic male patients, especially with elevation of systolic blood pressure.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1999年第3期174-177,共4页
Chinese Journal of Internal Medicine
基金
国家自然科学基金
上海市医学领先专业重点学科基金
上海市科委"科技启明星计划"(97QB14021)资助
关键词
糖尿病
高血压
瘦素受体
Ⅱ型
代谢紊乱
Diabetes mellitus
non insulin dependent
Hypertension
Gene
Receptor
leptin
