摘要
目的:观察人参皂苷Rg1诱导的神经干细胞(NSCs)在移植治疗缺氧缺血新生模型鼠中的作用。方法:体外用人参皂苷Rg1诱导分化NSCs,然后将诱导分化的NSCs移植入缺氧缺血的新生鼠模型侧脑室,采用TTC染色和行为学观察对模型进行评价。通过水迷宫、体感诱发电位观测其脑功能的恢复情况,免疫组化检测移植的NSCs生长、分化状况。结果:移植Rg1诱导后的NSCs,可以明显改善水迷宫的潜伏期、游泳路程、目标象限探索时间以及体感诱发电位的潜伏期和振幅,并在海马区域呈集中表达并围绕缺血损伤区域生长。结论:Rg1诱导后的NSCs移植在治疗新生大鼠缺氧缺血性脑损伤中发挥了较好的作用。
Objective:To observe the effect of Rg1-induced NSCs in treatment of neonatal rat model with hypoxiaischemia.Method:The neonatal rat model of HIE was established and assessed by using TTC staining and behavioral observation,then Rg1-induced NSCs was transplanted into the neonatal rat of HIE by lateral ventricle injection.Water maze test and somatosensory evoked potential were detected to observe brain function and the immunohistochemistry was done to assess growth and differentiation about transplanted NSCs a month after transplanted.Result:The transplantation of Rg1-induced NSCs could significantly shorten incubation period,swimming distance,exploration time of target quadrants of water maze test and incubation period and amplitude of somatosensory evoked potentials.Additionally,the concentrated expression appeared in the hippocampus and grew around the ischemic injury area in transplantation group.Conclusion:Transplantation of Rg1-induced NSCs play a better role in the treatment of neonatal HIE rats.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第4期509-514,共6页
China Journal of Chinese Materia Medica
基金
重庆市自然科学基金项目(cstc2011jjA10031)
关键词
人参皂苷RG1
神经干细胞
缺氧缺血性脑病
移植
ginsenosides Rg1
neural stem cells
hypoxic ischemic encephalopathy
transplantation
