摘要
目的探究人参皂苷Rg1(G-Rg1)促智信号转导途径,观察G-Rg1对环腺苷酸(cAMP)、磷酸化cAMP反应元件结合蛋白(p-CREB)和磷酸二酯酶(PDE)的影响。方法正常成年大鼠口服G-Rg120mg.kg-1,连续3d后,分别采用酶联免疫法和免疫组织化学法检测脑区cAMP和p-CREB水平的变化。体外给予G-Rg1后,检测海马匀浆液中PDE活性的变化。结果大鼠口服G-Rg1后,海马组织cAMP水平升高,海马和脑皮质p-CREB增强,G-Rg1降低海马PDE活性,抑制cAMP降解。结论G-Rg1能提高脑内cAMP、p-CREB水平,抑制PDE活性,表明G-Rg1可以通过激活cAMP-PKA-CREB信号通路发挥促智作用。
Aim To elucidate the nootropic signal transduction of ginsenoside Rgl （ G-Rgl ） by investigating its effects on cAMP, p-CREB and PDE. Methods Rats were given G-Rgl （20 mg · kg^-1） intragastri- cally for three days, the levels of cAMP and p-CREB were detected with enzyme-linked immuno assay and immunohistochemistry method, respectively. The influence on PDE activity was assayed in hippocampus homogenate in vitro. Results The levels of cAMP in hippocampus and p-CREB in hippocampus and cerebral cortex increased significantly in the rats treated with G-Rgl, and, simultaneously, the activity of PDE was definitely inhibited, leading to raise of cAMP levexert its nootropic action through cAMP-PKA-CREB signal pathway.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第6期740-743,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30672501)
