摘要
目的:通过兔单肺通气模型,观察长时间单肺通气后肺灌洗液中TNF-α、IL-8浓度的变化。方法:选择新西兰大白兔35只,分为对照组(C组)、双肺通气组(TLV组)和单肺通气组(OLV组),每组5只兔。TLV组和OLV组按通气时间1、3、5h分为3亚组,随后恢复双肺通气30min。对照组只行实验操作而不通气。观察通气时的心率(HR)、平均血压(MAP)、血气分析值、支气管肺泡灌洗液(BALF)中TNF-α、IL-8浓度。结果:与通气开始相比,OLV组在通气5hHR增高、MAP降低(P<0.01),pH、PaO2、SaO2在单肺通气后降低,PaCO2在OLV5h后升高(P<0.01);TLV组HR、MAP、pH、PaCO2在各观察时点无统计学差异(P>0.05)。OLV组左右肺的TNF-α和IL-8值在通气5h增高(P<0.01);TLV组的左肺的TNF-α和IL-8值在通气5h增高(P<0.01);OLV组通气5h左肺TNF-α、IL-8值与TLV组左肺比较有显著差异(P<0.01)。结论:单肺通气时肺损伤程度与单肺通气时间成正比,单肺通气肺损伤比双肺通气肺损伤严重。
Objective To observe the changes of the concentrations of TNF-α and IL-8 in bronchoalveolar lavage fluid（BALF） in rabbits after long time one-lung ventilation.Methods Thirty-five New Zealand white rabbits were divided into control group（Group C,n = 5）,two-lung ventilation group（Group TLV,n = 15）,and one-lung ventilation group（Group OLV,n = 15）.According to the time of ventilation,Group TLV and OLV were then divided into 3 sub-groups（1 h group,3 h group,and 5 h group,each n = 5）.Group C received experimental operation but without ventilation.Heart rate（HR）,median blood pressure（MAP）,the value of blood gas analysis,and the concentrations of TNF-α and IL-8 in BALF were measured.Results As compared with the basic value,HR and PaCO2 were increased,while MAP,pH,PaO2,and SaO2 were decreased in 5 h group of Group OLV（P 0.01）.But there were no significant changes of HR,MAP,pH and PaCO2 in Group TLV（P 0.05）.Concentrations of TNF-α and IL-8 in left and right lung were increased in 5 h group of Group OLV（P 0.01）;those in left lung were increased in 5 h group of Group TLV（P 0.01）;and those in left lung in 5 h group of Group OLV were significantly different from those in left lung in 5 h group of Group TLV（P 0.01）.Conclusion Lung injury in one-lung ventilation is severer than that in two-lung ventilation,and was proportional to the time of ventilation.
出处
《实用医学杂志》
CAS
北大核心
2012年第4期561-563,共3页
The Journal of Practical Medicine
基金
广州市卫生局一般引导项目立项资助(编号:201102A213127)
关键词
肺通气
炎症因子
肺灌洗液
Pulmonary ventilation
Inflammatory cytokines
Bronchoalveolar lavage fluid
