摘要
目的比较２种不同成份肺灌洗液的肺保存效果。方法将犬分成２组，一组供肺采用改良的ＥｕｒｏＣｏｌｉｎｓ液（含高钾，钾浓度１０８ｍｍｏｌ／Ｌ）灌洗，另一组用低钾葡萄糖液（ＬＰＧ液，钾浓度４ｍｍｏｌ／Ｌ）灌洗。４℃保存６小时后行异体左肺移植，保留受体的右肺。术后１０分钟～７２小时定时阻断右肺动脉血流，采股动脉血作血液气体分析，并作病理检查。结果改良的ＥｕｒｏＣｏｌｉｎｓ液和ＬＰＧ液灌洗的肺移植后即刻受体动脉血氧分压（ＰａＯ２）分别为（１７．３±６．２）ｋＰａ和（１９．７±７．０）ｋＰａ，ＣＯ２分压（ＰａＣＯ２）分别为（５．２８±１．１７）ｋＰａ和（４．８±０．７）ｋＰａ，两组比较，Ｐ＞０．０５，移植后７２小时的ＰａＯ２分别为（１４．６±６．１）ｋＰａ和（１８．４±７．６）ｋＰａ，ＰａＣＯ２分别为（４．２±０．９）ｋＰａ和（４．６±０．７）ｋＰａ，两组比较，Ｐ＞０．０５。移植肺组织光镜和电镜观察发现，灌洗后即刻及保存６小时的组织学改变主要表现为肺泡上皮及毛细血管上皮细胞肿胀、肥大、变性，移植后７２小时可见肺泡内有渗出、淋巴细胞浸润及血管壁增厚，２个组的病理改变相似。结论含高钾的改良ＥｕｒｏＣｏｌｉｎｓ液及含低钾?
Objective To compare the effects of the lung preservation with two different formula lung flushing solutions. Methods A newly canine left lung allotransplantation model was used. Twenty dogs were divided into two groups. Two types of flushing solutions were used: group A received modified Euro Collins solution(E C solution, K + 108mmol/L), group B low potassium glucose solution(LPG solution, K + 4mmol/L). After the pulmonary artery of donor dogs was flushed by E C solution or LPG solution, the allograft was stored at 4℃ for 6h. Then the left lung was transplanted into the recipient dog. Lung function and histological changes were studied immediately and 6, 12, 24, 48 and 72h after transplantation. Inflatable cuffs were placed around right pulmonary artery to permit the independent study of the transplanted lung.Results The E C solution and LPG solution provided similar immediate and 6～72h function of the preservation lung. Histological findings of the two groups were also very similar.Conclusion Potassium concentration in flushing solution is not the key factor to affect the effect of lung preservation.
出处
《中华器官移植杂志》
CAS
CSCD
1999年第1期53-55,共3页
Chinese Journal of Organ Transplantation
关键词
器官保存
肺移植
肺灌洗液
Lung Irrigation Potassium Organ preservation
