摘要
目的 :探讨口腔粘膜上皮角朊细胞 (KC)和细胞因子在口腔粘膜下纤维性变 (OSF)发生中的作用。方法 :分别从正常及OSF组织培养出KC(NM KC和OSF KC) ,用放射免疫法和酶联免疫吸附法测定培养细胞上清液中内皮素(ET)和β1转化生长因子 (TGFβ1)的水平。结果 :OSF KC分泌ET和TGFβ1水平均高于NM KC(P <0 .0 5 ) ,且两者呈正相关关系。结论 :外源性刺激导致的KC活化及其分泌的ET和TGFβ1的改变可能在OSF发生中起重要作用。
Objective: To assess the significance of oral mucosa keratinocytes (KCs) and cytokines in the development of oral submucous fibrosis (OSF). Methods: KCs were obtained and cultured from the buccal mucosa of healthy subjects who did not chew areca nut and patients with OSF who chewed areca nut. Then radioimmunoassay and enzyme linked immunosorbent assays were employed, and endothelin (ET) and transforming growth factor β\-1(TGFβ\-1) were quantified in culture supernatants. Results: The mean levels of ET and TGFβ\-1 secreted by OSF KC were 96.983±17 802 pg/ml and 4661 641±783 893 pg/ml respectively, which were higher than these secreted by NM KC (P<0 05); There was a positive correlation between the levels of ET and TGFβ\-1 secreted by OSF KC. Conclusion: External stimuli such as areca nut components may induce the development of OSF by activating and stimulating the keratinocytes to secret a series of cytokines, including ET and TGFβ\-1. It is speculated that ET and TGFβ\-1 may be the contributing factors in the pathological features noted in OSF.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2000年第1期23-25,共3页
West China Journal of Stomatology
关键词
口腔粘膜
纤维性变
角朊细胞
内皮素
TGFΒ1
oral submucous fibrosis\ \ keratinocytes\ \ endothelin\ \ transforming growth factor β\-1
