摘要
目的研究选择性环氧化酶-2（COX-2）抑制剂NS-398对长一中波紫外线诱导的永生化人类角质形成细胞（HaCaT）白介素-1a（IL-1a）、a肿瘤坏死因子（TNF-a）表达的影响，探讨NS-398对人类皮肤光损伤的保护作用机制。方法将传代培养的HaCaT细胞分为单纯照光组、NS-398干预组和空白对照组。单纯照光组用长-中波紫外线（UVA-UVB）照射，NS-398干预组预先用不同浓度NS一398处理后接受长一中波紫外线照射，空白对照组常规条件下培养不做处理。用酶联免疫吸附测定法（EusA）分别检测各组细胞上清液中IL-1a、TNF-d的表达。结果单纯照光组IL-1a、TNF-a表达量明显高于空白对照组；NS-398干预组IL-1a、TNF-a表达量明显低于单纯照光组，且呈NS-398浓度依赖性。结论NS-398能够抑制紫外线照射下HaCaT细胞IL-1a、TNF-a表达，从而可能对人类皮肤光损伤具有保护效应。
Objective 1o study the efiect of selective CC）X-2 inhibitor NS-398 on IL-la and TNF-a expression in HaCaT cells induced by UVA/UVB, and further to explore the mechanism on anti human skin photo-damage. Methods The subcultured HaCaT cells were divided into three groups： simple illuminated group was exposed to UVA/UVB directly, NS-398 interfered group was exposed to UVA/UVB after being treated with NS-398 in different concentrations, and the control group was cultured in normal without any treatment. The expression of IL-la and TNF-a in superna- tant was detected by ELISA kit. Results The level of IL-la and TNF-a expression in supernatant from simple illuminated group was remarkably higher than that in the control group, NS-398 inter- fered group showed much lower level than the simple illuminated one, and the expression of IL-la and TNF-a was dependent on the concentration of NS-398. Conclusions NS-398 can reduce IL-la and TNF-a expression in HaCaT cells induced by ultraviolet rays, suggesting the possibility of anti human skin photo-damage effect.
出处
《中华医学美学美容杂志》
2012年第1期52-55,共4页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
2008年湖北省科技计划自然科学基金一般项目（编号：2008CDB171）
