摘要
提出了气相色谱-质谱法测定蜂蜡净油中限用成分含量的方法。样品经二氯甲烷-三氯甲烷(1+1)混合溶剂溶解,采用不同极性的两根色谱柱分离后,选择离子监测模式下质谱法进行测定。共鉴定出96种化合物,其中香豆素和马索亚内酯是禁用成分。苯甲醇、肉桂醇、香豆素、马索亚内酯、苯甲酸苄酯、水杨酸苄酯和肉桂酸苄酯等7种限用物质的质量浓度在0.08～80mg.L-1范围内呈线性关系,检出限(3S/N)在0.01～0.23mg.kg-1之间。样品加标回收率在86%～101%之间,相对标准偏差(n=6)小于4.0%。
A method for determination of restricted ingredients in beeswax absolute by GC-MS was proposed.The sample was dissolved with mixed solvent of CH2Cl2-CHCl3（1＋1）,separated on two chromatographic columns with different polarity,and determined by MS under select ion monitoring（SIM） mode.Ninety-six components were identified,among which the coumarin and mossoia lactone were included to restricted ingredients.Linearity ranges between values of peak area and mass concentration of 7 restricted ingredients,including benzoic alcohol,cinnamic alcohol,coumarin,mossoia lactone,benzyl benzoate,benzyl salicylate and benzyl cinnamate,were obtained in the same range of 0.08-80 mg·L-1,with detection limit（3S/N） in the range of 0.01-0.23 mg·kg-1.Values of recovery found by standard addition method were in the range of 86%-101%,with RSD′s（n=6） less than 4.0%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2012年第1期61-64,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家烟草专卖局增香保润重大专项资金资助项目(No.110200902020)
