摘要
[目的]研究中华秋海棠根茎中甾醇的超声提取工艺条件。[方法]使用超声波提取法对中华秋海棠进行提取,利用改进的Liebermann-Bur chard比色法对秋海棠根茎超声波提取物中的甾醇进行定量分析,研究了料液比、温度、时间和提取溶剂4个因素对秋海棠根茎中甾醇含量的影响,选取提取温度、提取时间和料液比为影响因子,采用3元2次旋转正交设计,对超声提取工艺进行优化,计算回归方程,并进行响应面分析,同时采用高效液相色谱法分析其甾醇种类。[结果]回归方程准确可靠,且拟合情况良好。通过对回归方程进行局部寻优分析,得到甾醇提取的最佳工艺条件为:提取温度58℃,提取时间57 min,料液比1:44(g/ml);在此条件下,中华秋海棠中甾醇含量为1.80 mg/g。高效液相色谱分析显示,中华秋海棠含有β-谷甾醇及其他种类甾醇。[结论]超声波辅助提取法能提高提取效率,是一种节能、省时的好方法,作为中华秋海棠根β-谷甾醇提取的新工艺,具有良好的应用前景。
[Objective] To study the ultrasonic extraction technology of sterol from the rhizome of Begonia grandis Dry.subsp.sinensis（A.DC.） Irmsch..[Method] In this study,the sterol was first extracted from the rhizome of B.grandis using ultrasonic technology,and then quantitatively analyzed using the advanced Liebennann-Burchard colorimetric method.The single factor experiment and the orthogonal experiment were carried out to study the effects of factors including extraction temperature,extraction time and solid-liquid ratio on the yield of sterol.The regression equations to three factors were established by RSREG software in SAS8.1.In addition,the types of sterols were analyzed using HPLC.[Result] Results show that the regression equation was significant,the optimal technological conditions of sterol extraction were as follows：extracting temperature was 58℃,extracting time was 57 min,solid-liquid ratio was 1∶44 g/ml.Under these conditions,the content of sterol was 1.80 mg/g in the rhizome of B.sinensis.[Conclusion] Ultrasonic-assisted technology is an efficient,energy-saving and time-saving method that can be used to extract sterol from the rhizome of B.grandis.In addition,the analysis of HPLC spectrum reveals that sterol composition in the rhizome of B.sinensis is complex,including β-sitosterol and other sterols.
出处
《安徽农业科学》
CAS
2012年第6期3274-3277,3403,共5页
Journal of Anhui Agricultural Sciences
基金
国家"863"计划项目(2006AA10Z330)
