摘要
[目的]研究糯玉米自交系的遗传多样性,确定其亲缘关系远近,为糯玉米的新品种选育提供依据。[方法]以84个糯玉米自交系为试验材料,利用分布在玉米全基因组上的71个SSR标记对供试材料的遗传多样性进行分析,并对其产量及农艺性状与SSR标记进行了关联分析。[结果]150对SSR引物中有71对在糯玉米自交系中能扩增出多态性条带,共检测到342个等位基因,每对引物可检测到2～11个数目不等的等位基因,多态性信息量为0.249～0.876;糯玉米自交系间的遗传距离为0.02～0.32,均值为0.178,4个自交系可划分为8个组别;玉米10个连锁群上71个SSR位点的2 485个组合,都存在一定程度的连锁不平衡;共检测出21个SSR座位与10个产量、农艺性状显著相关。[结论]该试验阐明了所选糯玉米自交系的遗传多样性及相互间的亲缘关系,为有目的的组配糯玉米杂交种及其新品种选育提供了参考。
[Objective]To study the genetic diversity of waxy maize inbred lines and determine their genetic relationship,so as to provide references for the new varieties breeding of waxy maize.[Method] A total of 84 waxy corn inbred lines were used as experimental materials.Seventy one SSR markers in maize 10 chromosomes were adopted to explore the genetic diversity of waxy maize.The association of the agronomic and yield traits of waxy corn with the SSR primers was analyzed.[Result]Of the 150 SSR primers,71 could obtain polymorphic bands among waxy corn inbred lines.Seventy one pairs of primers in maize 10 chromosomes detected 342 alleles.The number of detected alleles was within a range of 2-11.Polymorphism information content（PIC） value ranged from 0.249 to 0.876,the genetic distance among the inbred lines was 0.02-0.32 with an average of 0.17;84 waxy maize inbred lines could be divided into 8 groups.The 2 485 combinations of 71 SSR primers at 10 linkage group were in linkage disequilibrium state;21 SSR markers were detected to be associated with 10 agronomic and yield traits.[Conclusion]The research expounded the genetic diversity of waxy maize inbred lines and their genetic relationship,which provided references for the hybrids and new varieties breeding of waxy maize.
出处
《安徽农业科学》
CAS
2012年第6期3212-3217,3283,共7页
Journal of Anhui Agricultural Sciences
基金
教育部"作物功能基因组重点实验室"开放课题及江苏省科技支撑计划(农业BE2011303)资助
关键词
糯玉米
遗传多样性
关联分析
农艺性状
产量性状
Waxy maize
Genetic diversity
Association analysis
Agronomic traits
Yield traits
