摘要
在植物的系统与进化研究中,相对于叶绿体和核基因片段而言,线粒体基因片段由于其系统发育信息位点少、结构复杂而较少应用到系统学研究中。本研究选用nad1基因的第二内含子序列重建玉蜀黍属主要种的系统发生树,在分子水平上验证了糯玉米在玉蜀黍属中系统学位置,并在此基础上探讨了nad1基因内含子序列的系统学意义及其在系统发育重建中的价值。结果表明,目前对玉蜀黍属内各类型材料的系统分类是合理的,糯玉米和玉米种内各类型材料之间遗传差异小;线粒体nad1基因第2内含子序列在种内和种间的变异较小,在种以上分类研究中具有一定的系统学价值。
In the study of plant systematics and evolution, the gene sequences of mitochondrial genome are less used for their low informative sites and complex structure compared to those of the nuclear and chloroplast genomes. In the study, we sequenced the intron 2 of mitochondrial nad1 gene to study the phylogeny of Genus Zea, in particular emphasiz the systematic position of waxy corn and discuss the value of the intron in the phylogenetic study. The results indicated that the division of the Genus Zea is correct and the distance in Zea mays between waxy corn and other maize types is close. The sequences of intron 2 can not provide enough information at the level of species in Zea mays, thus may be appropriate for the phylogenetic study at higher taxonomic.
出处
《四川农业大学学报》
CSCD
2007年第1期39-43,共5页
Journal of Sichuan Agricultural University
基金
教育部长江学者与创新团队资助计划项目(IRT0453)。