摘要
野生稻是水稻抗褐飞虱基因的重要种质资源。应用东乡野生稻与栽培稻协青早B构建的2套材料,开展水稻抗褐飞虱基因鉴定研究。先以协青早B//协青早B/东乡野生稻BC1F5群体为材料,应用褐飞虱田间种群进行抗虫鉴定,检测到2个抗褐飞虱QTL,其中,qBph2位于水稻第2染色体RM29-RG157区间,东乡野生稻等位基因可降低死苗率22.2%;qBph7位于第7染色体RM11-RM234区间,东乡野生稻等位基因可降低死苗率43.7%。进一步以协青早B为轮回亲本,构建了BC3F3群体,应用褐飞虱生物型I、II和III进行抗虫鉴定,QTL分析表明qBph2抗褐飞虱生物型I和II,qBph7抗褐飞虱生物型I和III。这2个QTL对培育抗褐飞虱水稻品种具有重要应用价值。
Wild species of Oryza is an important resource of the genes for resistance to brown planthopper（BPH）.In this study,two populations were developed from an interspecific cross between Dongxiang wild rice（Oryza rufipogon Griff.） and cultivated rice Xieqingzao B（Oryza sativa L.） and used to determine quantitative trait loci（QTLs） for BPH resistance.A BC1F5 population derived from Xieqingzao B//Xieqingzao B/Dongxiang wild rice was infested with BPH collected from the paddy field.Two QTLs were detected,of which qBph2 was located in the interval RM29-RG157 on chromosome 2 and qBph7 in the interval RM11-RM234 on chromosome 7,and the Dongxiang wild rice allele decreased seedling mortality by 22.2% and 43.7%,respec-tively.Validation of the two QTLs was followed by testing a BC3F3 population with BPH biotypes I,II,and III,respectively.QTL analysis showed that qBph2 confers resistance to biotypes I and II,while qBph7 to biotypes I and III.The two QTLs have a great potential in the improvement of BPH resistance of rice varieties.
出处
《作物学报》
CAS
CSCD
北大核心
2012年第2期210-214,共5页
Acta Agronomica Sinica
基金
国家自然科学基金项目(30671278)
中国超级稻项目(2011-6)
水稻生物学国家重点实验室自主研究课题(ZZKT200801)
关键词
东乡野生稻
褐飞虱
生物型
数量性状座位
Dongxiang wild rice
Brown planthopper
Biotype
Quantitative trait locus
