摘要
普通野生稻是栽培稻的祖先种,其遗传多样性远远大于栽培稻,蕴涵着栽培品种中难以找到的重要性状。以江西东乡普通野生稻为供体、以桂朝2号为遗传背景的野生稻基因渗入系(BC4F5、BC4F6)为材料,利用30%的PEG人工模拟干旱环境,对渗入系二叶一心苗期进行抗旱鉴定,共定位了12个与抗旱有关的QTL,其中在第2、6和12染色体上发现了4个QTL的加性效应值为正,来自东乡野生稻的等位基因能使渗入系的抗旱性增强,特别是位于第12染色体RM17附近的qSDT12-2在多次重复中均被检测到,在PEG处理后1-8d能稳定表达。通过对抗旱性QTL的动态分析,发现不同QTL表达时间不同。
Common wild rice （Oryza rufipogon Griff.） is the ancestor of cultivated rice （O.sativa L.）, which has a greater genetic diversity and important traits that remain to be employed in cultivated rice. In this study, a set of introgression lines （BC4F5 and/or BC4F6） carrying various introgressed segments from common wild rice, collected from Dongxiang county, Jiangxi Province, China,in the background of an Indica （O. sativa L. ssp. indica） cultivar, Guichao 2, was used. A total of 12 drought-related quantitative trait loci （QTL） were identified by investigating drought tolerance of introgression lines under 30% PEG treatment at the young seedlings stage. Of these QTLs, the alleles of 4 QTLs on chromosome 2, 6 and 12 from Dongxiang common wild rice were responsible for increased drought tolerance of the introgression lines. In particular, a QTL qSDT12-2, near RM17 on chromosome 12, was consistently detected in different replications, and expressed stably under PEG stress throughout the study. It was also found that the QTLs located on different chromosomes might express at different stages.
基金
This work was supported by the National Basic Research Program of China (No.2003CB114301),a grant from Protection and Utilization of Agricultural Wild Plants Program of the Ministry of Agriculture of China,and Chinese National Programs for High Technology Research and Development ("863" Program)(No. 2003AA207040).
关键词
普通野生稻
渗入系
苗期
PEG
抗旱性
QTL
common wild rice （Oryza rufipogon Griff.）
introgression lines
seedlings
PEG
drought tolerance
quantitative trait loci （QTL）
