摘要
目的研究波棱瓜愈伤组织诱导、分化及生根的条件,探讨波棱瓜快速繁殖新途径,为波棱瓜组织培养与离体植株再生奠定基础。方法以波棱瓜幼嫩茎段和叶片为材料,比较不同外植体、基本培养基及植物生长调节剂对波棱瓜愈伤组织诱导、分化及生根的影响。结果幼嫩茎段作为外植体时,其出愈率较叶片高,愈伤量大;MS基本培养基能够产生明显的愈伤组织,出愈率较高;幼嫩茎段愈伤组织诱导适宜的培养基为MS+6-BA2 mg/L+NAA0.5 mg/L,出愈率达93.3%。愈伤组织的最佳分化培养基为MS+6-BA2 mg/L+NAA0.1 mg/L+KT 3.0 mg/L,分化率达87.2%。生根培养的适宜培养基为1/2MS+NAA 1.0 mg/L,生根率达95%。结论波棱瓜幼嫩茎段在适宜的培养基中可通过愈伤组织途径分化不定芽,且极易生根,能够得到正常生长发育的再生植株。
Objective To investigate the conditions influencing the induction, differentiation, and rooting of callus in Herpetospermum pedunculosum, establish a new method for rapid propagation, and lay a foundation for plant tissue culture and regeneration. Methods With the young stems and leaves ofH. pedunculosum as materials, effects of different explants, basic medium, and plant growth regulators on callus induction, differentiation, and rooting were compared. Results When young stems were used as explants, the induction rate was higher than that of leaves. MS medium could produce obvious callus with high induction rate. The optimum compositions for callus induction, differentiation, and rooting were MS＋6-BA 2 mg/L＋NAA 0.5 mg/L, MS＋6-BA 2 mg/L＋NAA 0.1 mg/L＋KT 3.0 mg/L, and 1/2 MS＋NAA 1.0 mg/L. The callus induction rate, differentiation rate, and rooting rate were 93.3%, 87.2%, and 95%, respectively. Conclusion The young stems ofH. pedunculosum could differentiate the adventitious buds in a suitable medium through callus, and be easy to take root. The regeneration plantlets with normal growth and development could be obtained.
出处
《中草药》
CAS
CSCD
北大核心
2012年第2期360-363,共4页
Chinese Traditional and Herbal Drugs
基金
西藏自治区科技厅项目(2010KJGX01-36)
关键词
波棱瓜
离体培养
愈伤组织诱导
分化
植株再生
Herpetospermum pedunculosum （Ser.） Baill.
in vitro cultivation
callus induction
differentiation
plant regeneration
