摘要
目的:建立通脉丸中竹节香附素A及总皂苷含量测定方法。方法:采用HPLC法测定竹节香附素A的含量,色谱条件:AgilentSB-AQ色谱柱,乙腈与0.1%磷酸水为流动相梯度洗脱,流速0.8ml/min,检测波长206nm;采用比色法,以竹节香附素A为对照品,5%香草醛-冰乙酸-60%硫酸为显色剂,在531nm处测定吸光度,计算总皂苷的含量。结果:液相法测定竹节香附素A对照品质量在0.573～3.820μg(R2=0.9996)范围内呈良好线性关系,平均加样回收率为99.63%(RSD=1.05%);比色法测定竹节香附素A对照品质量在0.0242～0.2178mg范围内与吸光度呈良好线性关系(R2=0.9990),平均加样回收率为100.05%(RSD=1.69%)。结论:建立了通脉丸中竹节香附素A及总皂苷含量测定方法,此方法简便、准确、稳定,为其制剂的质量控制及新制剂的二次开发提供技术支持。
Objective： To establish a method for the determination of Raddeanin A and total saponins in Tongmai pill. Methods： A HPLC determination of Raddeanin A, Chromatographic conditions： Agilent SB-AQ chromatographic column, Gradient elution by Acetonitrile and 0.1% phosphoric acid water as mobile phase,velocity 0.8 ml/min, wavelength was 206 nm; Colorimetric method used to Raddeanin A as reference, 5% vanillin-acetic acid-60% sulfuric acid as the color reagent, absorbance was measured at 531 nm to calculate the total saponin content. Results： Determination of liquid Reference standard of Raddeanin A quality was linear in the range of 0.573-3.820 μg （R2=0.999 6）. The average recovery was 99.63% （RSD=1.05%）; Colorimetric method determination Raddeanin A quality was linear in the range of 0.024 2-0.217 8 mg （R2=0.999 6）. The average recovery was 100.05% （RSD =1.69%）. Conclusion： Established Tongmai pill of Raddeanin A and total saponins method for the determination , this method is simple, accurate, stable, for quality control and preparation of the secondary development of new agents to provide technical support.
出处
《中国当代医药》
2012年第1期5-7,共3页
China Modern Medicine
基金
河南省科技厅自然科学项目(102102310185)
河南省教育厅科技攻关项目(2011A360003)
关键词
通脉丸
竹节香附素A
总皂苷
含量测定
Tongmai pill
Raddeanin A
Total saponins
Content determination
