摘要
目的以超高效液相色谱质谱联用法(UPLC-MS/MS)快速鉴定乔松素在人肝微粒体中的代谢产物。方法以人肝微粒体体外孵育体系对乔松素进行代谢研究,以UPLC-MS/MS鉴定乔松素的体外代谢产物。结果建立了乔松素的体外代谢孵育体系,UPLC-MS/MS在6 min内检测到乔松素的4个代谢产物,分别鉴定为柚皮素、5,6,7-三羟基二氢黄酮、5,7,8-三羟基二氢黄酮和乔松素-7-葡萄糖醛酸苷,这4个代谢产物均为首次发现的乔松素人肝微粒体体外代谢产物。结论乔松素在人肝微粒体中的主要代谢途径为羟基化和葡萄糖醛酸化,5,7,8-三羟基二氢黄酮是其主要羟基化产物,乔松素-7-葡萄糖醛酸苷可能为乔松素的主要代谢失活形式。
OBJECTIVE To characterize the metabolism of pinocembrin in human liver microsome by UPLC-MS/MS. METHODS Pinocembrin was incubated with human liver microsomal incubation system. UPLC-MS/MS was used to characterize the metabolites. RESULTS Naringenin, dihydrobaicalein, dihydronorwogonin and pinocembrin-7-O-glucuronide were identified within 6 min via UPLC-MS/MS. These 4 metabolites were first reported as the metabolites of pinocembrin in human liver microsomal incubation system. CONCLUSION The results indicated that pinocembrin can only be metabolited as the forms of hydroxylation and glucnronidation in human liver microsome. Dihydronorwogonin was the main hydroxylated metabolite. Pinocembrin-7-O-glucuronide was the predominant deactivated metabolic pathway.
出处
《中国现代应用药学》
CAS
CSCD
2012年第2期159-163,共5页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省教育厅资助项目(Y200909605)
