摘要
酶法分离大豆(Glycine max L.)植株的幼荚子叶和无菌苗子叶的原生质体。用 K8P原生质体培养基悬滴培养,K8培养基加液。1个半月形成愈伤组织,转入固体 K8培养基中增殖,用 MSB(MS 无机成分+B5有机成分+0.5—1mg/l 2,4-D+0.2—0.5mg/l BA)培养基诱导瘤状愈伤组织的形成,再转入分化培养基 M_1(MS 无机成分+B5有机成分+0.5mg/l TZ+0.5mg/l BA+0.5mg/lKT+0.15mg/l NAA+500mg/l CH)中分化出苗。在 M_2培养基(1/2MS+0.2mg/l IBA)中生根,分化频率为13.6—24.2%,移栽盆中开花结荚。有两个大豆品种的幼荚子叶原生质体培养再生植株。
Protoplasts were isolated enzymatically from immature cotyledons of soybean.The protop- lasts divided to form calli in the K8P liquid medium.The calli further grew to 2-3 mm on the solid K8 medium and were transferred onto the MSB medium (MS minerals+B5 or- ganic components+0.5-1.0 mg/l 2,4-D+0.2-0.5 mg/l BA) to obtain compact and nodular calli. Shoot formation was initiated on M1 medium (MSB medium with 0.15 mg/l NAA,and BA,KT and ZT,0.5 mg/l of each,500 mg/l CH).Differentiation frequency was 13.6-24.2%.Plants have been regenerated from protoplasts of immature cotyledons in 2 cultivars,and normal pods were obtained from them.
关键词
大豆
原生质体
植株再生
Soybean(Glycine max L.)
Protoplasts
Plant regeneration
