摘要
本试验用阿特拉津溶液涂抹、荧光诱导动力学检测、分子杂交等方法对抗阿特拉津转基因大豆植株的后代进行了鉴定,在第二代及第三代中检测到了抗性基因的存在,表明从龙葵中得到的此抗阿特拉津 psbA 基因不仅能导人大豆叶绿体基因组中获得表达,而且可以遗传到后代。
The identification of the progenies of atrazine-resistante transgenic soybeen plants was carried out by using the methods of spraying the leaves directly with atrazine solution,ex- amining the change of leaf fluorescence kinetics curve under a brighter light induction and molecular hybridization.The resistant gene has been found in plants of F_2 and F_3 generations, it means that the atrazine-resistance psbA gene from black nightshade not only can be trans- ferred to the chloroplast genome of atrazine-susceptible soybean and expressed,but also inherit- ed to the progenies.
关键词
大豆
阿特拉津
转基因植株
Atrazine
psbA gene
Transgenic plant
Soybean
Black nightshade
