摘要
从土壤中分离到一株高产中性植酸酶的菌株,酶活力达12.52U/mL。对其进行形态、生理、生化、分子生物学鉴定,初步鉴定为放射型根瘤杆菌(Agrobacterium radiobacter)。酶学性质研究结果表明:该酶的最适反应温度为45℃;最适反应pH值为7.0;65℃处理60min酶活力保持80%左右,有一定耐热性;在pH5.5～8.0之间,稳定性较好;Ba2+对酶活力有一定的激活作用,Fe2+、Mg2+、Zn2+和Cu2+对酶活力均有一定程度的抑制作用,其中Fe2+的抑制作用最强。
A high-yield neutral phytase-producing strain with an enzymatic activity of 12.52 U/mL was isolated from soil and initially identified as Agrobacterium radiobacter based on its morphological,physiological and biochemical characteristics as and 16S rDNA sequence.The optimum reaction temperature and pH of neutral phytase produced by the strain were 45 ℃ and 7.0,respectively.The relative activity of the enzyme was 80% after heating at 65 ℃ for 60 min,revealing that it was thermostable to some extent.The enzyme was stable at pH values in the range of 5.5-8.0.Its activity could be activated by Ba2＋ but inhibited by Fe2＋,Mg2＋,Zn2＋ and Cu2＋.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第1期120-124,共5页
Food Science
基金
江苏省高校自然科学基础研究面上项目(10KJD180006)
徐州工程学院科研基金青年课题(XKY2009122)
徐州工程学院科研基金培育课题(XKY2010112)
徐州工程学院大学生创新创业基金项目(201112)
关键词
中性植酸酶
筛选
鉴定
酶学性质
neutral phytase
screening
identification
enzymatic properties
