摘要
以继代培养一年后的水飞蓟(Silybum marianum Gaerta.)叶愈伤组织为材料进行了原生质体的分离和培养的研究。在 M_(12)培养基中培养3天后观察到第一次分裂,最高分裂频率为35.4%,1—3个月后得到肉眼可见的小愈伤组织,将增殖长大的愈伤组织转至 D_6分化培养基后出现绿色芽点的分化。水飞蓟幼苗下胚轴切段接种于 MS+NAA 0.8mg/l、6-BA0.5mg/1、水解酪蛋白200mg/1 的培养基上诱导出愈伤组织,转至 D_6培养基后分化苗的频率可达75%,再转入生根培养基得到了根的分化,再生植株生长旺盛,移栽工作正在进行之中。
Leaf calli of Silybum marianum Gaertn.subcultured for one year were used for proto- pIast isolation and culture.First division was observed three days after culture on medium M12,and the highest division frequency was 35.4%.One to three months later,small ralli were seen with naked eyes,and grew up gradually.Upon transferring them onto D6 tifferentiation medium,the green bud apices were observed two months later.However,no shoot differentiation was obtained. Hypocotyl calli were induced on MS+NAA 0.8mg/l,6-BA 0.5mg/l.Two months after transferring calli onto D6 medium,shoots were regenerated from the surface of the calli. The freqency of shoot differentiation was 75%.On a MS rooting medium containing NAA 0.5mg/l,IBA 0.1 mg/l,whole plants with healthy roots were obtained.
关键词
水飞蓟
原生质体培养
愈伤组织
Silybum marianum Gaertn.
Protoplast Culture
Tissue culture
Plant regeneration
