摘要
采用RAPD分子标记技术,对黑龙江省境内收集的24份野生桑树种质资源进行遗传多样性分析,揭示其遗传分化关系,以利于对耐寒、抗旱类型野生桑树种质资源保存与应用价值的准确鉴定和评估。从20个RAPD随机引物中筛选出4个有效引物,对24份野生桑树种质材料的基因组DNA进行扩增,共获得275条带,其中多态性带273条,多态性比率为99.3%,单引物对每份种质材料扩增获得的条带数在2～5条之间。24份野生桑树种质材料的遗传距离在0.047～1.000之间,其中,杜蒙2号和杜蒙3号,宁安1号和宁安3号两组种质材料的相似性最大,杜蒙3号和宁安3号的相似性最小。24份野生桑树种质材料的亲缘关系与其地域分布基本吻合。
In order to facilitate an accurate identification and evaluation to the preservation and practical value of wild mulberry germplasm resources on their cold tolerance and drought resistance,genetic diversity and phylogenetic relationship among 24 wild mulberry germplasm resources collected from Heilongjiang Province was analyzed by random amplified polymorphic DNA（RAPD） molecular marker technology.From 20 RAPD random primers,4 effective primers were obtained.Amplification to genomic DNAs of these 24 wild mulberry germplasm materials yielded 275 bands,among which 273 were polymorphic.The polymorphic rate reach 99.3%.And the number of obtained bands from amplifying single mulberry germplasm material with single primer ranged from 2 to 5.Genetic distance between the 24 wild mulberry germplasm materials was within the range of 0.047 to 1.000.Among them,the highest genetic resemblance was between Dumeng 2 and Dumeng 3,and between Ning’an 1 and Ning’an 3.And the lowest genetic resemblance was between Dumeng 3 and Ning’an 3.The genetic relationship among 24 wild mulberry germplasm materials was basically in agreement with their regional distribution.
出处
《蚕业科学》
CAS
CSCD
北大核心
2012年第1期8-13,共6页
ACTA SERICOLOGICA SINICA
基金
黑龙江省教育厅海外学人重点项目(No.1153h05)
关键词
桑树种质资源
耐寒类型
RAPD标记
遗传多样性
Mulberry germplasm resource
Cold-tolerant ecotype
RAPD marker
Genetic diversity
