摘要
目的：观察缺氧预适应（ＰＣ）对体外培养的心肌细胞的保护作用以及不同时相的 ＰＣ对心肌细胞摄取２０１ＴＩ的影响。材料和方法：在原代培养的乳鼠心肌细胞基础上，建立早期缺氧预适应（ＥＰ）和晚期缺氧预适应（ＤＰ）模型，观察ＥＰ和 ＤＰ条件下心肌细胞存活和摄２０１Ｔｌ情况。结果：与缺氧／复氧组相比较， ＥＰ组细胞存活率提高 ２０％， ＤＰ组提高１２％，均有显著差异（Ｐ＜０．０１）。ＥＰ组心肌细胞在复氧回小时后对２０１ＴＩ的摄取接近正常组（Ｐ＞０．０５），ＤＰ组心肌细胞在复氧１小时后‘’‘ＴＩ的摄取量低于正常组（Ｐ＜０ ０５），与缺氧／复氧组相比无显著性差异（Ｐ＞０ ０５）。结论：ＥＰ和ＤＰ能够保护体外培养乳鼠心肌细胞，两时相摄取２０１ＴＳＩ表现不同，可能与其不同的保护机制有关。
Purpose: To study the protection on cultured cardiomyocytes of hypoxic preconditioning (HPC) and to investigate the effects at different time of HPC on Thallium-201 uptake in vitro. Materials and Methotls: In the early protection (EP) and delayed protection (DP) models, the effects of HPC on the viabilty of primary cultured neonatal rat cardiomyocytes and thallium-201 uptake were observed. Results: Compared with normal contral group, the viable myocytes in EP group increased 20 % and in DP increased 12% (P<0.01). The uptake of thallium-201 in group of EP reached normal level (P>0.05) at for after reoxygenation, while that in group of DP kept low (P <0.05) in comparison with normal contral group and similar to the uptake at the group of hypoxia/reoxygenation (P>0.05). Conclu non: EP and DP of HPC existed in primary cultured neonatal rat cardiomyocytes and the effects at the two phases on the uptake of thallium-201 were different, which may be related to their different protective mechanisms.
出处
《中国医学影像学杂志》
CSCD
2000年第1期51-53,共3页
Chinese Journal of Medical Imaging
基金
陕西省自然科学基金!98SM44
关键词
心肌保护
缺氧预适应
细胞摄取
铊
myocardial protection
hypoxic preconditioning
celluar uptake
thallium
