摘要
为提高发酵产量,根据裂殖壶菌生物合成二十二碳六烯酸(DHA)的途径,考察添加代谢途径关键酶的辅酶及酶的抑制剂对发酵裂殖壶菌的影响。结果表明:添加生物素可促进油脂积累,添加浅蓝菌素有利于DHA及不饱和脂肪酸含量的提高。添加生物素0.3 mg/L时,DHA占细胞干质量分数达11.26%,相对于对照提高了13%;当添加浅蓝菌素0.1mg/L时,DHA占细胞干质量分数可达12.2%。
To improve the DHA productivity,effects of biotin and cerulenin addition on DHA production were investigated based on DHA synthesis pathway of Schizochytrium sp.The results showed that the biotin addition could enhance the lipid accumulation,while the cerulenin addition could increase DHA and polyunsaturated fatty acid content.The percentage of DHA in cell dry weight reached 11.26% at 0.3 mg/L biotin addition,thus it was 13% higher than that of the control.When 0.1 mg/L cerulenin was added,DHA content reached 12.2%.
出处
《生物加工过程》
CAS
CSCD
2012年第1期42-45,共4页
Chinese Journal of Bioprocess Engineering
基金
江苏省高校产业化推进项目(JH09-30)
江苏省六大高峰人才项目(2008-D-63)
教育部新世纪优秀人才支持计划资助项目(NCET-09-0157)
霍英东教育基金资助项目(123014)
