摘要
目的 :探讨急性非淋巴细胞白血病 ( ANL L )多药耐药基因 ( MDR1 )与免疫表型表达的关系及意义。方法 :应用反转录聚合酶链反应 ( RT- PCR)和间接免疫荧光法对 5 2例初治 ANL L 患者进行了 MDR1 基因及免疫表型的检测。结果 :ANL L 患者 MDR1 阳性表达率为 3 0 .77% ,其中 M3 明显低于其他亚型。 MDR1 阳性 ANL L 患者 CD3 4 表达率显著高于 MDR1 阴性者 ;而其完全缓解率 ( 3 7.5 % )则低于后者 ( 88.89% )。完全缓解 ( CR)率与 CD3 4的表达有明显相关性 ( P<0 .0 1)。 MDR1 与 CD3 4 同时表达则 CR率 ( 2 5 % )明显降低。结论 :MDR1 与 CD3 4 的表达在初诊 ANL L 患者中均具有重要的预后意义。 MDR1 + / CD+ 3 4的 ANL L
Objective:To explore the relationship and significance between MDR 1 and immunophenotype in ANLL. Methods: Reverse transcriptase polymerase chain reaction and indirect immunofluorescence assay were used to detect the expression of MDR 1 and immunophenotype in 52 patients with de novo ANLL.Results: The positive percentage of MDR 1 in 52 patients was 30.77%. The positivity of MDR was lower in M 3(1/18) than that in non M 3. The CD 34 expression rate of MDR 1 positive patients (75%) was significantly higher than that of MDR 1 negative (22.22%) patients. CR rate was significantly differ between MDR + 1 patients(37.5%) and MDR - 1 patients (89.89%)( P <0.01).CR rate was correlated with CD 34 expression ( P <0.01). CR rate was lower than that of other conditions in patients with MDR + 1/CD + 34 . Conclusion: It was suggested that the expression of both MDR 1 and CD 34 had prognostic significance in ANLL. ANLL patients with MDR + 1/CD + 34 predict a poor prognosis.
出处
《白血病》
2000年第1期19-21,共3页
关键词
白血病
非淋巴细胞
急性
免疫分型预后
Leukemia
Nonlymphoblastic
Acute Immunophenotype
Prognosis
