摘要
目的:测定粉花月见草中总黄酮的含量。方法:以无水乙醇作为回流提取溶剂,在375nm处用紫外可见分光光度法测定粉花月见草中总黄酮的含量。结果:测得粉花月见草中总黄酮的含量为1.4435mg/g,精密度RSD=0.14%(n=5),回收率为90.87%~98.98%。结论:该方法操作简便、准确、可靠,可用于植物中总黄酮的含量测定。
Objective: To determine the content of total flavonoids in Oenothera rosea.Methods: Ethanol is applied as the extracting solvent,at 375nm with UV-visible spectrophotometry to determine the content of total flavonoids in Oenothera rosea.Results: The content of total flavonoids 1.4435mg / g,the precision RSD = 0.14%(n = 5),the rate of recovery 90.87%~98.89%.Conclusion: The method is simple,accurate,reliable,and can be applied to determine the content of total flavonoids in plants.
出处
《黔南民族师范学院学报》
2011年第6期1-3,共3页
Journal of Qiannan Normal University for Nationalities
基金
贵州省教育厅2010年度自然科学研究基金资助项目(黔教科2010094)
贵州省黔南民族师范学院2009年度重点资助科研项目(QNSY0915)
关键词
粉花月见草
山奈酚
紫外分光光度法
含量测定
oenothera rosea
kaempferol
UV-visible spectrophotometry
the determination of content