摘要
目的了解慢性肾功能衰竭(CRF)患者外周血T淋巴细胞亚群的变化规律,探讨CRF患者的免疫功能状态。方法采用三色流式细胞学检测技术,检测34例CRF患者,34例非CRF患者和194例健康体检者的外周血T淋巴细胞亚群水平,并对其结果进行比较分析。结果 CRF组总T细胞、T4细胞、T8细胞的百分含量(%)依次为62.69±2.00、35.69±1.38和20.90±1.58;T4/T8比值2.19±0.24,总T细胞、T4细胞、T8细胞的绝对含量(×109/L)依次为0.88±0.08、0.50±0.05和0.30±0.03。与非CRF组和对照组比较发现,患者总T细胞、T4细胞、T8细胞绝对含量的减少(P<0.01,P<0.05),总T细胞、T8细胞在淋巴细胞中所占比例的减少(P<0.01,P<0.05),T4/T8比值升高(P<0.01)。结论 CRF患者机体免疫功能紊乱,且以T8细胞功能减低和T4细胞功能相对亢进为特征。流式检测CRF患者T淋巴细胞亚群有助于病情和疗效监测。
Objective To study the alteration regularity of peripheral blood T-lymphocyte subsets in patients with chronic renal failure（CRF group）,and investigate their immune functional status.Methods Peripheral blood T-lymphocyte subsets were detected by 3-color flow cytometry in 34 patients with CRF,34 patients with non-CRF（non-CRF group） and 194 health people（control group）.The results were analyzed statistically.Results In CRF group,the percentages of T lymphocyte,CD4＋T lymphocyte and CD8＋T lymphocyte as well as the ratio of CD4＋/CD8＋ were 62.69±2.00,35.69±1.38,20.90±1.58 and 2.19±0.24,and the absolute quantities of T lymphocyte,CD4＋T lymphocyte and CD8＋T lymphocyte were 0.88±0.08、0.50±0.05 and 0.30±0.03.Compared with non-CRF group and control group,the absolute quantities of T-lymphocyte,CD4＋T lymphocyte and CD8＋T lymphocyte were significantly lower（P0.01,P0.05）,and the ratio of CD4＋/CD8＋ was clearly higher（P0.01）.Conclusion The results suggest that the function of CD8＋T-lymphocyte decrease,of CD8＋T-lymphocyte rise,and there is immune function disorder in patients with CRF.Peripheral blood T-lymphocyte subsets in patients with CRF detected by flow cytometry conduce to patients＇ condition and curative effect monition.
出处
《国际检验医学杂志》
CAS
2011年第20期2317-2318,2320,共3页
International Journal of Laboratory Medicine
