摘要
【目的】研究棉花细胞质雄性不育(CMS)系与保持系线粒体基因组的差异,为克隆棉花CMS基因奠定基础。【方法】以atpA,atp6,atp9,ccmB,ccmC,ccmFN1,cob,coxI,coxII,coxIII,matR,nad1bc,nad2,nad4,nad5,nad6,nad7c,nad9,rpl5,rrn18等20个线粒体基因保守序列为探针,利用Southern blot方法对棉花细胞质雄性不育系、保持系线粒体基因组进行RFLP分析。【结果】发现atpA、atp9、ccmB、nad1bc、nad6、nad7c、rrn18等基因在棉花不育系和保持系间存在明显RFLP多态性,其中atpA的差异最明显。【结论】atpA是棉花CMS系和保持系线粒体基因组间的一个明显差异位点。CMS系比保持系缺失一个rrn18拷贝。
【Objective】 Identification of the differences of mitochondrial genomes between cytoplasmic male sterile（CMS） line and maintainer line of cotton could make a foundation for cloning CMS-associated gene in cotton.【Method】In this study,20 mitochondrial gene probes,atpA,atp6,atp9,ccmB,ccmC,ccmFN1,cob,coxI,coxII,coxIII,matR,nad1bc,nad2,nad4,nad5,nad6,nad7c,nad9,rpl5,and rrn18,were used to identify the differences between the P30B normal fertile（N-） and the P30A male sterile（S-） cytoplasm with Southern blot method.【Result】Among the 20 genes,atpA,atp9,ccmB,nad1bc,nad6,nad7c and rrn18 revealed restriction fragment length polymorphisms（RFLPs） between N-and S-cytoplasm.The differences in atpA locus were most obvious.【Conclusion】 atpA was an important mitochondrial genomic site that showed an obvious difference between CMS line and maintainer line of cotton.One rrn18 gene copy was deleted in the mitochondrial genome of CMS line.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第2期208-217,共10页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30771371)
关键词
棉花
细胞质雄性不育
线粒体基因组
RFLP
ATPA
cotton（Gossypium hirsutum L.）
CMS（cytoplasmic male sterility）
mitochondrial genome
RFLP
atpA
