摘要
用毛细管胶束电动色谱成功分离并测定了高粱和玉米中7种农药类环境激素(多菌灵、西玛津、莠去津、毒死蜱、溴氰菊酯、乙草胺和氯氰菊酯)的残留量。研究了电泳缓冲液及表面活性剂等因素的影响,在最佳分离条件(pH 9.0、20 mmol/L磷酸氢二钠+50 mmol/L SDS+5%乙腈为缓冲溶液,分离电压20 kV,检测波长222 nm,实验温度25℃,压力法进样,30 mbar×10 s)下,7种农药在28 min内得到基线分离,质量浓度与其峰面积在5.0～150μg.L-1范围内呈良好线性,检出限为0.6～3.0μg/L,回收率为97%～108%,相对标准偏差为2.2%～4.7%。该方法具有操作简单、快速方便及自动化程度高、重现性好等优点。
A method was developed for the simultaneous separation and determination of carbendazim,simazine,atrazine,chlorpyrifos,decamethrim, acetochlor and cypermethrin in corn and durra by micellar electrokinetic capillary electrophoresis.The conditions for separation and detection of a mixture of seven pesticides were optimized.The results showed that,under the optimized conditions of running buffer：20 mmol/L Na2HPO4＋50 mmol/L SDS＋5% acetonitrile（pH 9.0）,separation voltage：20 kV,detection wavelength：222 nm,ambient temperature：25 ℃,pressure injection：30 mbar×10 s,the analytes were successfully separated within 28 min.The calibration curves of seven pesticides were linear in the range of 5.0-150 μg·L-1.The detection limits were in the range of 0.6-3.0 μg/L.This method was successfully applied in the analysis of seven pesticides in corn and durra samples with spiked recoveries of 97%-108% and RSD of 2.2%-4.7%.
出处
《分析测试学报》
CAS
CSCD
北大核心
2012年第1期40-44,共5页
Journal of Instrumental Analysis
基金
内蒙古自然科学基金项目资助(2009MS0310)
关键词
胶束电动色谱
农药
分析
环境激素
micellar electrokinetic capillary electrophoresis
pesticides
analysis
endocrine-disrupting chemicals
