摘要
目的:建立HPLC同时测定血栓心脉宁片中芦丁与丹酚酸B含量的方法。方法:选用AgilentHC-C18(4.6mm×150mm,5μm)色谱柱,流动相为甲醇-乙腈-0.5%甲酸(30∶9∶61),流速为0.7mL·min-1,检测波长为257nm。结果:丹酚酸B在0.207～2.07μg与峰面积呈良好线性(r=0.9996),平均回收率为98.6%,RSD=1.0%(n=6);芦丁在0.2028～2.028μg与峰面积呈良好线性(r=0.9999),平均回收率为99.5%,RSD=0.5%(n=6)。结论:该方法简便、准确、重复性好,可用于血栓心脉宁片中芦丁与丹酚酸B含量测定,以控制该制剂的质量。
Objective：To establish an new assay method for rutin and Salvianolic acid B in xueshuan xinmaining tablets by HPLC. Methods： The AgilentHC-C18 column （4. 6 mm × 150 mm,5 μm） was adopted with a mobile phase of Methanol-acetonitrile -0. 5% formic acid（30： 9：61 ）, the flow rate was 0. 7 mL · min^-1 , the wavelength of detection was set at 257 rim. Results： Salvianolic acid B＇s linearity was obtained within the range of 0. 207 -2. 07 txg （r =0. 999 6）, and the average recovery（n = 6） was 98.6 %, and RSD was 1.0 %. Rutin＇s linearity was obtained within the range of 0.2028 -2.028μg （r =0.999 9）, and the average recovery（n =6） was 99.5 %, and RSD was 0.5 %. Conclusion： The method is simple, reliable and reproducible, and can be used to control the quality of xueshuanxinmaining tablets.
出处
《中国现代中药》
CAS
2011年第12期43-45,共3页
Modern Chinese Medicine
