摘要
目的研究血浆样品经热灭活(56℃30min)后,其艾滋病病毒(HIV)抗体筛查和确证试验结果是否会受到影响。方法取5份HIV抗体阳性血浆样品,各分为2管,其中1管进行热灭活处理,然后对这2组样品分别进行10倍系列稀释,用1种HIV抗体酶联免疫吸附试验(ELISA)试剂检测,比较S/CO比值。取300份血浆样品,分为灭活组和普通组,分别用5种HIV抗体ELISA试剂(其中第三代试剂3种、第四代试剂2种)和3种HIV抗体快速检测试剂进行筛查检测,出现筛查阳性反应的样品进一步做确证试验。结果随着稀释度的增加,5份HIV抗体阳性样品灭活前后的ELISA检测结果(S/CO比值)都逐渐减小,直至转为阴性反应,其中4份样品灭活后比灭活前早一个稀释度转阴,1份同时转阴,提示灭活过程会略微降低HIV抗体的浓度。用5种ELISA试剂、3种快速检测试剂检测300份样品,样品灭活前后的检测结果差异均无统计学意义;对出现筛查阳性反应的所有样品,灭活前后的确证试验检测结果一致。结论经56℃30min热灭活后,尽管HIV阳性血浆样品中的HIV抗体浓度略有降低,但对于未经稀释的常规临床血浆样品来说,热灭活处理不会明显影响目前常用筛查、确证试剂的检测结果。
Objective To study the effect of using heat-inactivated（56 ℃,30min） plasma specimens with several HIV antibody screening and confirmatory assay kits.Methods Five HIV antibody-positive plasma specimens were aliquoted into 2 vials,respectively.One group of them was heat-inactivated by 56 ℃,30min.Both groups were 1∶10 serially diluted and then detected by an ELISA kit for HIV antibody.Three hundred plasma specimens were aliquoted into 2 vials,respectively,and one group of them was heat-inactivated.Both groups were detected by 5 ELISA kits（including 3 HIV antibody and 2 HIV antigen/antibody ELISAs） and 3 HIV rapid tests,respectively.The repeat-reactive specimens were further detected with one HIV antibody confirmatory assay,HIV Blot 2.2 WB（MP Biomedicals Asia Pacific Pte.Ltd.,Singapore）.Results For all the specimens from both groups of 1∶10 dilution series,the S/CO ratios gradually decreased and then became negative with the dilution process.Compared with the normal group,the dilution specimens became negative earlier in 4 of the 5 heat-inactivated series,indicating that the heat-inactivation process slightly decreased the concentration of HIV antibody.However,for the 300 normal clinical plasma specimens,there were no statistically significant difference between the heat-inactivated and normal groups by using 5 HIV ELISA kits and 3 HIV rapid tests,and the confirmatory results of repeat-reactive specimens between 2 groups were concordant.Conclusions The heat-inactivation（56 ℃,30min） on normal plasma specimens will not significantly affect the detection results by using current HIV antibody screening and confirmatory assay kits,although this process may slightly decrease the concentration of HIV antibody.
出处
《中国艾滋病性病》
CAS
2011年第6期618-620,628,共4页
Chinese Journal of Aids & STD
关键词
艾滋病病毒
筛查试验
确证试验
热灭活
HIV
Screening assays
Confirmatory assays
Heat-inactivation
